Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,564,912 |
G→A |
36.2% |
intergenic (‑124/+168) |
yfdE ← / ← glk |
predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,564,912 | 0 | G | A | 36.2%
| 31.4
/ 30.5
| 37 | intergenic (‑124/+168) | yfdE/glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base A (7/6); total (20/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564843‑1564978
|
aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:101632/70‑1 (MQ=255)
aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:3013231/70‑1 (MQ=255)
aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:1541799/70‑1 (MQ=255)
aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:2166967/70‑1 (MQ=255)
tCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAAc > 1:2315587/1‑70 (MQ=255)
tAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATc < 1:2391969/71‑1 (MQ=255)
aTGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa < 1:2080784/63‑1 (MQ=255)
gATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGt > 1:776499/1‑70 (MQ=255)
aaTTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaaa > 1:890099/1‑71 (MQ=255)
ttCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaata < 1:1854017/71‑1 (MQ=255)
ttCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaata < 1:2405714/71‑1 (MQ=255)
ttCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat > 1:1018333/1‑70 (MQ=255)
tCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat > 1:1242000/1‑69 (MQ=255)
tCGTTCAGTGATAGTCGCCGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATg > 1:2419965/1‑70 (MQ=255)
gTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataa > 1:2389746/1‑66 (MQ=255)
ttCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:671014/71‑1 (MQ=255)
tCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt > 1:798701/1‑70 (MQ=255)
cAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGtt > 1:2830269/1‑50 (MQ=255)
aGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:3297752/71‑1 (MQ=255)
gTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTa < 1:545573/69‑1 (MQ=255)
gttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGga < 1:2500423/65‑1 (MQ=255)
gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt < 1:1798497/70‑1 (MQ=255)
gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt < 1:59295/70‑1 (MQ=255)
aGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:181605/71‑1 (MQ=255)
aGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:3414564/71‑1 (MQ=255)
aGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc < 1:672981/71‑1 (MQ=255)
gTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaata > 1:1715010/1‑46 (MQ=255)
gTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGaa < 1:3849277/63‑1 (MQ=255)
gAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGc > 1:3922336/1‑71 (MQ=255)
gAAATTATGAACGTGCACAACAAATCGCCGTTACCAGACAAATAATGCAATTa > 1:2959777/1‑53 (MQ=39)
aaaTTATGAACGTGCAGAACAAATCGCCGTTACCa > 1:1156977/1‑35 (MQ=255)
aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACaa > 1:1382240/1‑40 (MQ=255)
aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:3588276/1‑71 (MQ=255)
aTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:351853/1‑70 (MQ=255)
aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAt > 1:2786445/1‑47 (MQ=255)
aTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg > 1:3761525/1‑70 (MQ=255)
ttATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa < 1:3687046/38‑1 (MQ=255)
gAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg < 1:3070986/70‑1 (MQ=255)
gTGCAGAAGAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATc > 1:3090924/1‑55 (MQ=255)
tGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:3607200/71‑1 (MQ=255)
gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt > 1:1011055/1‑70 (MQ=255)
|
AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGT > minE/1564843‑1564978
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
