Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
267,502 |
G→T |
P228H (CCT→CAT) |
ykfA ← |
predicted GTP‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 267,502 | 0 | G | T | 100.0%
| 151.7
/ NA
| 46 | P228H (CCT→CAT) | ykfA | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (33/13); total (33/13) |
AGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTA > W3110S.gb/267468‑267552
|
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:1029777/41‑1 (MQ=255)
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:79488/41‑1 (MQ=255)
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:3707303/41‑1 (MQ=255)
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:3875419/41‑1 (MQ=255)
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:1675320/41‑1 (MQ=255)
aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:1890241/41‑1 (MQ=255)
aGCCTGTACCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:3658979/41‑1 (MQ=255)
gCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:1499861/40‑1 (MQ=255)
ccTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGa < 1:3479199/39‑1 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:4499157/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:4313752/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:4219198/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:4157882/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:1613506/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:3849360/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:3769786/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:2499066/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:1426819/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:2781907/1‑41 (MQ=255)
tCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGa > 1:705014/1‑41 (MQ=255)
ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg < 1:1049612/61‑1 (MQ=255)
ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg < 1:2380460/61‑1 (MQ=255)
ggACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg < 1:1701395/61‑1 (MQ=255)
ccGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg < 1:1220318/55‑1 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:416105/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:701422/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:4638133/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:4303307/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:4246625/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:2188676/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:79992/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:3955873/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:3945614/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:2195548/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:2243006/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:3814025/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:277886/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:2797648/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcagg > 1:3470561/1‑55 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag > 1:2177501/1‑54 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag > 1:4647883/1‑54 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt > 1:946051/1‑60 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt > 1:1145826/1‑60 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa > 1:4400520/1‑61 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa > 1:2189894/1‑61 (MQ=255)
gCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa > 1:3128626/1‑61 (MQ=255)
|
AGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTA > W3110S.gb/267468‑267552
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
