Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
478,343 |
C→T |
A45T (GCT→ACT) |
ylaC ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 478,343 | 0 | C | T | 100.0%
| 19.1
/ NA
| 8 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (2/6); total (2/6) |
GGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAT > W3110S.gb/478308‑478382
|
ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGcccc < 1:2962212/61‑1 (MQ=255)
ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGcccc < 1:4399032/61‑1 (MQ=255)
aCATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:360322/62‑1 (MQ=255)
cACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa < 1:4697434/62‑1 (MQ=255)
cACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa < 1:698749/62‑1 (MQ=255)
ccGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAt > 1:3704145/1‑61 (MQ=255)
cAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTa < 1:2589618/52‑1 (MQ=255)
aaGTAACGTACATACCGATAAACAGCCCCGGATGTTTa > 1:129491/1‑38 (MQ=255)
|
GGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGAT > W3110S.gb/478308‑478382
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
