Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
922,613 |
G→A |
L615L (CTG→CTA) |
macB → |
fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 922,613 | 0 | G | A | 100.0%
| 14.5
/ NA
| 6 | L615L (CTG→CTA) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/6); total (0/6) |
TGGGAGATTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACC > W3110S.gb/922581‑922663
|
tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:1985482/62‑1 (MQ=255)
tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:3987379/62‑1 (MQ=255)
tGGGAGATTGGTTTTTCACCGTTGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCgg < 1:454343/62‑1 (MQ=255)
ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:140806/62‑1 (MQ=255)
ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3389420/62‑1 (MQ=255)
ttGGCGCTGCTACTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:454059/62‑1 (MQ=255)
gctactgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3207907/54‑1 (MQ=255)
gctactgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTAcc < 1:3665267/54‑1 (MQ=255)
|
TGGGAGATTGGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACC > W3110S.gb/922581‑922663
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
