Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,259,237 |
G→A |
S613F (TCT→TTT) |
tdcE ← |
pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,259,237 | 0 | G | A | 100.0%
| 90.4
/ NA
| 28 | S613F (TCT→TTT) | tdcE | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/16); total (12/16) |
GACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTT > W3110S.gb/3259190‑3259295
|
gacgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGa > 1:1313497/1‑62 (MQ=255)
gacTGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:3327063/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTc < 1:2704736/56‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTc < 1:3503958/56‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:464111/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:1316859/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:4398707/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:1821338/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:1952005/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:3728948/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:2469608/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:2568726/62‑1 (MQ=255)
gacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:2918369/62‑1 (MQ=255)
acCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:2881487/61‑1 (MQ=255)
acCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATc < 1:3960259/61‑1 (MQ=255)
gTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACt < 1:3078820/62‑1 (MQ=255)
gTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACt < 1:3626361/62‑1 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg > 1:3882362/1‑56 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg > 1:818034/1‑56 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg > 1:4303528/1‑56 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg > 1:1740700/1‑56 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCg > 1:1162317/1‑56 (MQ=255)
cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACtaga > 1:2172347/1‑59 (MQ=255)
ttACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGAc > 1:1167373/1‑62 (MQ=255)
ttttCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGtt > 1:4598436/1‑61 (MQ=255)
ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt > 1:499377/1‑62 (MQ=255)
ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt > 1:1846565/1‑62 (MQ=255)
ttAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCttt > 1:994701/1‑62 (MQ=255)
|
GACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTT > W3110S.gb/3259190‑3259295
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
