Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,064,477 |
A→G |
E374E (GAA→GAG) |
gntU → |
gluconate transporter, low affinity GNT 1 system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,064,477 | 0 | A | G | 100.0%
| 143.3
/ NA
| 39 | E374E (GAA→GAG) | gntU | gluconate transporter, low affinity GNT 1 system |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/18); total (21/18) |
CGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTC > W3110S.gb/4064432‑4064536
|
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:4168183/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:2908937/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:1546833/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:3201746/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:1614985/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:2672918/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:1769418/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:2124922/62‑1 (MQ=255)
cGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCg < 1:239965/62‑1 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATggcggc > 1:3158092/1‑60 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc > 1:2174476/1‑62 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc > 1:2423998/1‑62 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc > 1:3360209/1‑62 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc > 1:1722121/1‑62 (MQ=255)
ggTAGGACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGgcgc > 1:1581616/1‑62 (MQ=255)
gACTGGTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCg > 1:3317852/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:3621327/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:3599537/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:3594279/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:4118301/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:1210412/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:2308827/1‑62 (MQ=255)
gTGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg > 1:1017852/1‑62 (MQ=255)
tGATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGt > 1:2394148/1‑62 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:305742/60‑1 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:2471929/60‑1 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:2450749/60‑1 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:3893525/60‑1 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:4214082/60‑1 (MQ=255)
gATGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:4138785/60‑1 (MQ=255)
aTGCCGGTTATTGAGCGACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTAt > 1:306494/1‑62 (MQ=255)
aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:2657948/59‑1 (MQ=255)
aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTg < 1:399174/59‑1 (MQ=255)
aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTAt > 1:984788/1‑62 (MQ=255)
aTGCCGGTTATTGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGAAt > 1:746012/1‑62 (MQ=255)
ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt > 1:1410817/1‑62 (MQ=255)
ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt > 1:3985472/1‑62 (MQ=255)
ttGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTggtggt > 1:290662/1‑62 (MQ=255)
tGAGCAACTGAACTACTCCGGTGCGCAAATGGCGGCG‑‑GTCGATTTGTATCGCTGGTGGTTc < 1:904949/61‑1 (MQ=255)
|
CGTAGCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGTGCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTC > W3110S.gb/4064432‑4064536
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
