Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
753,855 |
T→C |
N189S (AAC→AGC) |
ycfT ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 753,855 | 0 | T | C | 100.0%
| 175.9
/ NA
| 48 | N189S (AAC→AGC) | ycfT | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/30); total (18/30) |
AGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAT > minE/753790‑753920
|
aGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCAtt > 1:2747419/1‑71 (MQ=255)
gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa > 1:2461497/1‑67 (MQ=255)
gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcc < 1:2469259/71‑1 (MQ=255)
gTCATTATTGTTGCGCCAAACCATGAGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa > 1:884416/1‑67 (MQ=255)
cATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTccc < 1:32383/70‑1 (MQ=255)
cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGt < 1:140788/70‑1 (MQ=255)
cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg < 1:659935/71‑1 (MQ=255)
ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTggg < 1:3125510/71‑1 (MQ=255)
ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTggg < 1:219108/71‑1 (MQ=255)
aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc > 1:1472163/1‑71 (MQ=255)
aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc > 1:2221380/1‑71 (MQ=255)
aCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc > 1:1091532/1‑70 (MQ=255)
ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGTATCACACTGCTCATTCCCCACCACGGCGTGGGAACg < 1:2204749/70‑1 (MQ=255)
ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg > 1:3055071/1‑64 (MQ=255)
ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACg < 1:1992672/70‑1 (MQ=255)
ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACg < 1:926536/70‑1 (MQ=255)
ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGa < 1:20248/71‑1 (MQ=255)
tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt < 1:1339763/71‑1 (MQ=255)
cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:66723/70‑1 (MQ=255)
cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:742541/70‑1 (MQ=255)
ccAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAAc > 1:3174133/1‑71 (MQ=255)
ccAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAAc > 1:3082/1‑71 (MQ=255)
aGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc < 1:1917549/71‑1 (MQ=255)
gCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCca > 1:1768077/1‑71 (MQ=255)
taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca < 1:1697704/69‑1 (MQ=255)
taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACt > 1:2713768/1‑71 (MQ=255)
taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACt > 1:70420/1‑71 (MQ=255)
taaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag < 1:2554921/71‑1 (MQ=255)
aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:1063048/1‑70 (MQ=255)
aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag > 1:2277420/1‑70 (MQ=255)
aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCAACACTcag > 1:2527419/1‑70 (MQ=255)
gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg < 1:2775621/39‑1 (MQ=255)
gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg < 1:838986/39‑1 (MQ=255)
gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg < 1:315378/39‑1 (MQ=255)
aaTTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTaca < 1:1691203/71‑1 (MQ=255)
ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGa > 1:1537721/1‑43 (MQ=255)
ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt < 1:3063682/46‑1 (MQ=255)
ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt < 1:3082322/46‑1 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:3143541/45‑1 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:1513547/45‑1 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:2863109/45‑1 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt < 1:915344/45‑1 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTa > 1:1430063/1‑46 (MQ=255)
gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTa > 1:766321/1‑46 (MQ=255)
ggATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag < 1:3021792/61‑1 (MQ=255)
ggATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag < 1:2868103/61‑1 (MQ=255)
tCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCg < 1:1837676/71‑1 (MQ=255)
cacTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAt < 1:991527/71‑1 (MQ=255)
|
AGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAT > minE/753790‑753920
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
