Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,954,781 |
Δ1 bp |
intergenic (‑69/‑99) |
ygfB ← / → zapA |
hypothetical protein/protein that localizes to the cytokinetic ring |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,954,778 | 0 | A | . | 98.1%
| 226.9
/ ‑1.4
| 52 | intergenic (‑66/‑102) | ygfB/zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (23/28); total (23/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 |
GACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAA > minE/1954708‑1954843
|
gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGATCAGa < 1:1480960/71‑1 (MQ=255)
gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:1198127/71‑1 (MQ=255)
gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:494125/71‑1 (MQ=255)
aCATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:38373/70‑1 (MQ=255)
aGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAggg < 1:3184963/70‑1 (MQ=255)
ttCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGt < 1:1605006/71‑1 (MQ=255)
tcgtcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGtct < 1:189370/69‑1 (MQ=255)
gtcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtcttc > 1:1692884/1‑71 (MQ=255)
tcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtcttct > 1:818812/1‑71 (MQ=255)
aaaGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGt < 1:780063/62‑1 (MQ=255)
atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:1311402/1‑71 (MQ=255)
atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:1587034/1‑71 (MQ=255)
atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:201860/1‑71 (MQ=255)
tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGtc > 1:526734/1‑52 (MQ=255)
tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:55309/1‑70 (MQ=255)
tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCt < 1:1521982/71‑1 (MQ=255)
atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:271759/1‑71 (MQ=255)
atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:35077/1‑71 (MQ=255)
atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:724894/1‑71 (MQ=255)
ttCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta < 1:1685112/70‑1 (MQ=255)
cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:572432/1‑71 (MQ=255)
cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:1458707/1‑71 (MQ=255)
cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:847866/1‑71 (MQ=255)
tGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg < 1:3001712/71‑1 (MQ=255)
tGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg > 1:2396308/1‑71 (MQ=255)
tACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtctt < 1:3134247/42‑1 (MQ=255)
tACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtctt < 1:3172603/42‑1 (MQ=255)
aCCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCtt > 1:2763911/1‑71 (MQ=255)
ccACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCt < 1:3049316/69‑1 (MQ=255)
cACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg < 1:610674/71‑1 (MQ=255)
cACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg > 1:637766/1‑71 (MQ=255)
aCTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGc < 1:2292204/71‑1 (MQ=255)
tttGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg > 1:2947152/1‑68 (MQ=255)
gCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGAcc < 1:123557/69‑1 (MQ=255)
gCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCAt < 1:355198/71‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGt < 1:939134/40‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:3023604/46‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:994595/46‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:1561526/46‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg < 1:2251848/71‑1 (MQ=255)
ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg > 1:2054687/1‑71 (MQ=255)
tggtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGc < 1:2678884/60‑1 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg > 1:501888/1‑46 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:2786317/69‑1 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:1866939/69‑1 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:646542/69‑1 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTc > 1:2225680/1‑70 (MQ=255)
gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTc > 1:2302270/1‑70 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGt > 1:1300626/1‑35 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:586973/71‑1 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:557496/71‑1 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:225327/71‑1 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:1408664/71‑1 (MQ=255)
tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:485620/71‑1 (MQ=255)
gACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTaaa < 1:1972610/71‑1 (MQ=255)
aCCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa > 1:464536/1‑68 (MQ=255)
|
GACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAA > minE/1954708‑1954843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
