Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,350,543 |
G→A |
35.8% |
V337I (GTA→ATA) |
nrdA → |
ribonucleoside diphosphate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,350,543 | 0 | G | A | 35.8%
| 51.9
/ 49.7
| 53 | V337I (GTA→ATA) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/11); new base A (11/8); total (34/19) |
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGT > W3110S.gb/2350476‑2350609
|
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1065548/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:648336/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:618788/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3240181/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3099931/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:3033023/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2853616/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1520467/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2427502/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2208521/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:2113075/1‑70 (MQ=255)
aaGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1740338/1‑70 (MQ=255)
aaGCCTGATGGTGTTGAAAAACAAACGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTa > 1:1375309/1‑70 (MQ=255)
gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:99629/70‑1 (MQ=255)
gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:909768/70‑1 (MQ=255)
gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATcaaca < 1:196137/70‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:3244698/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1876099/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:2054203/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1313772/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:1313785/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATg < 1:3025608/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGGACAAATCAACAAACTGATg < 1:1821087/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:1125077/71‑1 (MQ=255)
aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:525006/71‑1 (MQ=255)
aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:510650/70‑1 (MQ=255)
aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:386340/70‑1 (MQ=255)
aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:99244/70‑1 (MQ=255)
aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:3102732/70‑1 (MQ=255)
aaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:3221348/70‑1 (MQ=255)
acCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:106846/1‑71 (MQ=255)
acCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:203543/1‑71 (MQ=255)
ggtgTGGAAGGCAACCGCGTGAGTCATATGGACTACTGGATACAAATCAACAAACTGATg < 1:694110/60‑1 (MQ=255)
cgcgTGCGTCATATGGACTACGGGGTACAAATCATCAAACTGATg < 1:780904/45‑1 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:860538/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:3284816/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:3201338/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1372461/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:171940/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:2413328/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1722846/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGt > 1:1736324/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCAGTTCAGCCCGt > 1:2781667/1‑71 (MQ=255)
cGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGAGAAGATATCACCCTGTTCAGCCCg > 1:889038/1‑70 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTTTGCTGAAAGGTGAAGa > 1:2446576/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGa > 1:756845/1‑48 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1161723/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:3092838/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2966303/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2479814/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1096382/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:881951/1‑51 (MQ=255)
cGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1024731/1‑51 (MQ=255)
|
AAGCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTTCAGCCCGT > W3110S.gb/2350476‑2350609
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
