Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F3 I0 R2	260	44.3	3360226	90.5%	3041004	64.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,866,110	T→A	100%	intergenic (‑20/+160)	rpoS ← / ← nlpD	RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,866,110	0	T	A	100.0%	89.8 / NA	29	intergenic (‑20/+160)	rpoS/nlpD	RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/19); total (10/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC  >  W3110S.gb/2866072‑2866171

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:3279754/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:3187165/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:1202466/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:879555/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:1655534/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:1733003/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:2092366/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:823653/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:277760/1‑42 (MQ=255)

aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt                                                            >  1:2498908/1‑42 (MQ=255)

       ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg                         <  1:2442959/70‑1 (MQ=255)

       ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg                         <  1:3154670/70‑1 (MQ=255)

       ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg                         <  1:1161636/70‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:2573599/71‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:1002760/71‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:799775/71‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:3116012/71‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:1651926/71‑1 (MQ=255)

                             cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:1100539/71‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:2652143/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:751378/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:489962/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:3306462/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:3233956/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:3195321/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:2219710/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:2122994/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc                    <  1:1580494/52‑1 (MQ=255)

                              tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc  <  1:883641/70‑1 (MQ=255)

ACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC  >  W3110S.gb/2866072‑2866171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A