Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,682,573 |
G→A |
100% |
L211L (CTG→TTG) |
ilvD ← |
dihydroxyacid dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,682,573 | 0 | G | A | 87.5%
| 15.3
/ ‑3.3
| 8 | L211L (CTG→TTG) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (0/7); total (1/7) |
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGG > W3110S.gb/3682526‑3682623
|
ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:266147/71‑1 (MQ=255)
ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:2687433/71‑1 (MQ=255)
ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:2948144/71‑1 (MQ=255)
ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg < 1:3346384/71‑1 (MQ=255)
ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAg > 1:2459001/1‑71 (MQ=255)
cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg < 1:1209712/70‑1 (MQ=255)
cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg < 1:1636409/70‑1 (MQ=255)
cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg < 1:21604/70‑1 (MQ=255)
|
GGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGG > W3110S.gb/3682526‑3682623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
