Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
710,341 |
C→T |
P44S (CCA→TCA) |
ybfN → |
predicted lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 710,341 | 0 | C | T | 100.0%
| 14.4
/ NA
| 6 | P44S (CCA→TCA) | ybfN | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (1/5); total (1/5) |
AGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG > W3110S.gb/710301‑710391
|
agagGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCaga > 1:2299374/1‑65 (MQ=255)
ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag < 1:1709239/65‑1 (MQ=255)
ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag < 1:363392/65‑1 (MQ=255)
ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag < 1:742771/65‑1 (MQ=255)
ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag < 1:752836/65‑1 (MQ=255)
ccGCACAAGGTTCGTCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag < 1:821422/65‑1 (MQ=255)
|
AGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG > W3110S.gb/710301‑710391
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
