Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I2 R1 330 0.0 2544031 91.9% 2337964 62.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,000,742 C→T M292I (ATG→ATA)  yedO ← D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,000,7420CT100.0% 268.3 / NA 79M292I (ATG→ATA) yedOD‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/76);  total (3/76)

GAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAA  >  W3110S.gb/2000687‑2000770
                                                       |                            
gAATCGGCCCTTCATCTTTTAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:224357/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:595185/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:249796/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:2148015/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:216598/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:363121/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1841570/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1794500/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:2180291/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1759403/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1693488/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1680199/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:550804/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1557768/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:627123/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:640145/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:783165/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1254862/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:724332/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:706044/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1285286/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:681790/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:65213/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:641979/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1453865/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1539658/65‑1 (MQ=255)
gAATCGGCCCTTCATCTTTGAAGCGTTTCCGACTGATACCGTCAATCAGCCCCGCTATCGCtttt                     <  1:1461294/65‑1 (MQ=255)
    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:450028/65‑1 (MQ=255)
    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:446997/65‑1 (MQ=255)
    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:1678555/65‑1 (MQ=255)
    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:380971/65‑1 (MQ=255)
    cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg                 <  1:2024841/65‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:242939/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2341272/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:248383/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2401174/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:992587/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2512417/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2518427/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:307838/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:351004/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:445865/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:56819/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:811849/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:965743/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:102062/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1652818/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:102864/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1102265/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1163297/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1192634/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1279552/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1283200/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1349372/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1351824/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1445978/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1514829/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1552886/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:157945/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1612522/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2329245/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1757722/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1761006/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1822684/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1883543/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1932015/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1692/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:1940212/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:203424/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2121313/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2144242/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2166470/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2217181/64‑1 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:401205/1‑63 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:628020/1‑63 (MQ=255)
                    aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCa   >  1:633975/1‑63 (MQ=255)
                    aaGCGTTTCCGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:2299542/64‑1 (MQ=255)
                    aaGCGTTCCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCaa  <  1:954033/64‑1 (MQ=255)
                                               aGCCCCGCTATCGCTTTTCCGGTATTCACAGGATCaa  <  1:333692/37‑1 (MQ=38)
                                                       |                            
GAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAA  >  W3110S.gb/2000687‑2000770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A