Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,000,742 |
C→T |
13.1% |
M292I (ATG→ATA) |
yedO ← |
D‑cysteine desulfhydrase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,000,742 | 0 | C | T | 13.1%
| 103.8
/ 7.1
| 38 | M292I (ATG→ATA) | yedO | D‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (17/16); new base T (2/3); total (19/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 |
AATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000679‑2000810
|
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTTATACCGTCAATCAGCCCCGCTATCGc < 1:3211655/69‑1 (MQ=255)
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGc > 1:2470100/1‑69 (MQ=255)
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGc > 1:1861645/1‑69 (MQ=255)
aGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt > 1:2852552/1‑66 (MQ=255)
aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:600554/69‑1 (MQ=255)
aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:437617/69‑1 (MQ=255)
aaTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:300887/69‑1 (MQ=255)
aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGta < 1:2279421/69‑1 (MQ=255)
aTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt < 1:296592/68‑1 (MQ=255)
cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtata < 1:3110024/69‑1 (MQ=255)
cGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtata > 1:2767967/1‑69 (MQ=255)
cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGa > 1:2315981/1‑68 (MQ=255)
aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:3032896/43‑1 (MQ=255)
aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1596686/43‑1 (MQ=255)
aTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1128681/43‑1 (MQ=255)
tCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc < 1:1252645/42‑1 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg > 1:260310/1‑53 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATaca < 1:1580651/59‑1 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:3434878/1‑67 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:3440333/1‑67 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCa > 1:402427/1‑67 (MQ=255)
aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1139182/68‑1 (MQ=255)
aaGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1510414/68‑1 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCt > 1:2382947/1‑68 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATacac < 1:3523175/49‑1 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:2908034/60‑1 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1745024/61‑1 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCt > 1:1524361/1‑68 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAAACAGGATCAAGCAg < 1:3091697/61‑1 (MQ=255)
ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:1793047/49‑1 (MQ=255)
ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc > 1:2225875/1‑69 (MQ=255)
ccGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGcc > 1:223445/1‑69 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATTCACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:122907/1‑69 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1976641/45‑1 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg > 1:2457359/1‑45 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:2726101/45‑1 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:3021567/1‑69 (MQ=255)
aaTCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCaa > 1:2889490/1‑69 (MQ=255)
cAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt < 1:1325279/69‑1 (MQ=255)
aGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTc < 1:3529932/69‑1 (MQ=255)
aGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCa > 1:1381249/1‑64 (MQ=255)
aGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt > 1:1717552/1‑68 (MQ=255)
cATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc < 1:1877358/69‑1 (MQ=255)
cATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc < 1:2215065/69‑1 (MQ=255)
|
AATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000679‑2000810
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
