Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,000,742 |
C→T |
M292I (ATG→ATA) |
yedO ← |
D‑cysteine desulfhydrase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,000,742 | 0 | C | T | 100.0%
| 140.2
/ NA
| 47 | M292I (ATG→ATA) | yedO | D‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/20); total (27/20) |
ATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC > W3110S.gb/2000676‑2000804
|
aTGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg > 1:4397652/1‑71 (MQ=255)
tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg > 1:1661508/1‑70 (MQ=255)
tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg < 1:3095620/70‑1 (MQ=255)
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt > 1:3582472/1‑71 (MQ=255)
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt > 1:3089062/1‑71 (MQ=255)
aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt > 1:2808803/1‑71 (MQ=255)
aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCttt > 1:2794986/1‑71 (MQ=255)
aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTcc > 1:4624359/1‑71 (MQ=255)
ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATacac < 1:3944383/71‑1 (MQ=255)
ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATaca > 1:4462250/1‑70 (MQ=255)
tCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGtata > 1:1455673/1‑62 (MQ=255)
tCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGAt > 1:3744620/1‑70 (MQ=255)
cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATc > 1:74000/1‑70 (MQ=255)
cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATc > 1:2399458/1‑70 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGtata > 1:1084957/1‑57 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg > 1:2836950/1‑69 (MQ=255)
tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg > 1:21321/1‑69 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGc < 1:1836365/68‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:1698671/69‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:688326/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:3183691/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:3269558/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:3309837/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:3643009/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:3809496/70‑1 (MQ=255)
tGAAGCGTTTCTGACTGATACCGTCAATCAG‑‑CCGCTATCGCTTTTCCGGTATACACAGGATCAAGCa < 1:717044/67‑1 (MQ=255)
tttCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGaa > 1:3420277/1‑65 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc > 1:3004365/1‑70 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc > 1:3129558/1‑70 (MQ=255)
tCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTc > 1:4060271/1‑70 (MQ=255)
tgactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCgg < 1:2947228/40‑1 (MQ=255)
gactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAgg < 1:3828615/49‑1 (MQ=255)
gactgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAgg < 1:4135831/49‑1 (MQ=255)
actgaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:1711233/57‑1 (MQ=255)
tACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAg < 1:341443/52‑1 (MQ=255)
ccGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg < 1:3748257/54‑1 (MQ=255)
ccGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg < 1:1738901/54‑1 (MQ=255)
tCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGcc > 1:1311075/1‑53 (MQ=255)
aaTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt > 1:862417/1‑71 (MQ=255)
aaTCAGCCCCGCTATCGCTTTTCCGGTATACAAAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt > 1:3469743/1‑71 (MQ=255)
aTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAttt < 1:3507684/71‑1 (MQ=255)
tCACCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTc < 1:2487573/71‑1 (MQ=255)
cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa > 1:4095764/1‑71 (MQ=255)
cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa > 1:3845030/1‑71 (MQ=255)
cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa > 1:4647078/1‑71 (MQ=255)
cAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCa > 1:2101647/1‑71 (MQ=255)
aGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAc > 1:2050974/1‑71 (MQ=255)
|
ATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAC > W3110S.gb/2000676‑2000804
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
