Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,442,070 |
A→G |
T497A (ACG→GCG) |
thiC → |
thiamin (pyrimidine moiety) biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,442,070 | 0 | A | G | 100.0%
| 91.8
/ NA
| 26 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/9); total (17/9) |
CGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCT > W3110S.gb/3442011‑3442122
|
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:1466546/62‑1 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:1524640/62‑1 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:1905642/62‑1 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:1920983/62‑1 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:2205892/62‑1 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg < 1:219072/62‑1 (MQ=255)
ttGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCAt < 1:1960102/65‑1 (MQ=255)
cagcgcGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1683325/3‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCAt > 1:2055691/1‑37 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt > 1:2272772/1‑64 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:290474/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:688331/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:358231/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:372795/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:428933/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:432463/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:432957/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1052122/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:281852/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:2166715/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:2123102/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1911285/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa > 1:1378003/1‑65 (MQ=255)
cTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAAAAAAGAAGATGTTa > 1:523220/1‑65 (MQ=255)
ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt < 1:2356926/65‑1 (MQ=255)
ctctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt < 1:1834989/65‑1 (MQ=255)
|
CGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCT > W3110S.gb/3442011‑3442122
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
