Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,442,070 |
A→G |
18.4% |
T497A (ACG→GCG) |
thiC → |
thiamin (pyrimidine moiety) biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,442,070 | 0 | A | G | 18.4%
| 106.1
/ 19.4
| 49 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base G (2/7); total (21/28) |
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 |
ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATT > W3110S.gb/3442002‑3442138
|
aCCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACg > 1:1135255/1‑71 (MQ=255)
tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:194183/1‑69 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:2425015/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3422977/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3487759/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3706619/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3758739/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:4031233/1‑68 (MQ=255)
gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:1495588/71‑1 (MQ=255)
gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:2464096/71‑1 (MQ=255)
tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCa < 1:3371667/71‑1 (MQ=255)
tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCa < 1:2957858/71‑1 (MQ=255)
gATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTg > 1:2507763/1‑70 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGTTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg < 1:3788940/71‑1 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg > 1:3732918/1‑71 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg < 1:1366099/71‑1 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCT‑‑GCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:1249628/1‑71 (MQ=255)
ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCt > 1:1400025/1‑71 (MQ=255)
ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCt > 1:915467/1‑71 (MQ=255)
atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2094733/1‑70 (MQ=255)
atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2009242/1‑70 (MQ=255)
atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2004602/1‑70 (MQ=255)
ttGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:398588/71‑1 (MQ=255)
tGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCAt > 1:2644865/1‑49 (MQ=255)
cTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATg < 1:270778/71‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:1389619/40‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:3866531/40‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:2178243/40‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:1859788/40‑1 (MQ=255)
ggCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:1622368/70‑1 (MQ=255)
ggCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:626410/70‑1 (MQ=255)
gcgcGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:1834339/66‑1 (MQ=255)
tGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc < 1:565884/41‑1 (MQ=255)
tctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt < 1:820362/53‑1 (MQ=255)
tctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt < 1:756229/53‑1 (MQ=255)
ttACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtata < 1:1095693/71‑1 (MQ=255)
ttACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaag < 1:2898300/44‑1 (MQ=255)
ttACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:4004442/43‑1 (MQ=255)
tACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtatt < 1:2791400/70‑2 (MQ=255)
aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:261041/1‑43 (MQ=255)
aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:1423003/1‑43 (MQ=255)
aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:2056239/1‑43 (MQ=255)
aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:4045430/1‑68 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa < 1:1475069/45‑1 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:2574235/67‑1 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:1978463/67‑1 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:2709178/71‑1 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:4041536/71‑1 (MQ=255)
tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:502046/71‑1 (MQ=255)
|
ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATT > W3110S.gb/3442002‑3442138
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
