Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,442,070 |
A→G |
T497A (ACG→GCG) |
thiC → |
thiamin (pyrimidine moiety) biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,442,070 | 0 | A | G | 100.0%
| 232.2
/ NA
| 67 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/36); total (31/36) |
ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAG > W3110S.gb/3442002‑3442135
|
aCCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCg > 1:3152843/1‑71 (MQ=255)
cTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa < 1:1507238/71‑1 (MQ=255)
tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:3554079/1‑69 (MQ=255)
tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:2621726/1‑69 (MQ=255)
tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:2329165/1‑69 (MQ=255)
tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:4649158/1‑69 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:1660876/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:2496873/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:2452539/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:415071/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:3379668/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:3619189/1‑68 (MQ=255)
cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa > 1:3554171/1‑68 (MQ=255)
cGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAagag < 1:94099/71‑1 (MQ=255)
gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGc < 1:3969478/71‑1 (MQ=255)
tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCa < 1:3886991/71‑1 (MQ=255)
cGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCa < 1:3135122/70‑1 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTggg < 1:3615788/71‑1 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTggg < 1:3117073/71‑1 (MQ=255)
aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTggg > 1:3812905/1‑71 (MQ=255)
tGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTc > 1:1434695/1‑71 (MQ=255)
gcggcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGcc > 1:956228/1‑71 (MQ=255)
ggcgATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCaa < 1:713939/50‑1 (MQ=255)
gatgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAAt > 1:540980/1‑70 (MQ=255)
gatgatTGGCTGGTTTGGCGGCGCGATGCTCTGTTACGTAGCGCCAAAagag < 1:4445127/52‑1 (MQ=39)
gatgatTGGCTGGTTTAGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAAt > 1:3501713/1‑70 (MQ=255)
gatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:21757/1‑68 (MQ=255)
gatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:3141550/1‑68 (MQ=255)
atTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGt > 1:3544077/1‑57 (MQ=255)
ttGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:479098/71‑1 (MQ=255)
ttGGCTGGTTTGGCTGCGCGATGCTCTCTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:4090837/71‑1 (MQ=255)
tGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:4594987/70‑1 (MQ=255)
tccTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga < 1:2190995/69‑1 (MQ=255)
gCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTgg < 1:98829/51‑1 (MQ=255)
gCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga < 1:58397/70‑1 (MQ=255)
ccTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga < 1:3202061/69‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCa < 1:3995750/42‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCa < 1:2092997/42‑1 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt > 1:3423124/1‑70 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt > 1:1331219/1‑70 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt > 1:3306395/1‑70 (MQ=255)
gTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa < 1:3863546/71‑1 (MQ=255)
ttGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATc > 1:3120884/1‑42 (MQ=255)
ttGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATc > 1:127531/1‑42 (MQ=255)
cgcgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:2816582/70‑1 (MQ=255)
cgcgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAgggg < 1:4094809/70‑1 (MQ=255)
gcgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt < 1:3343549/71‑1 (MQ=255)
gcgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt < 1:4486789/71‑1 (MQ=255)
gcgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt < 1:4241395/71‑1 (MQ=255)
cgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAATATGTTAAGCAGGGGCtt < 1:645237/71‑1 (MQ=255)
cgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCtt < 1:1909540/71‑1 (MQ=255)
cgATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCt > 1:3730550/1‑70 (MQ=255)
cgATGCTCTCTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCtt < 1:1245180/71‑1 (MQ=255)
tGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATc < 1:4014880/71‑1 (MQ=255)
gCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATg > 1:1121747/1‑53 (MQ=255)
gCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCa > 1:4113719/1‑71 (MQ=255)
ttACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat < 1:2324573/70‑1 (MQ=255)
ttACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCta < 1:152278/69‑1 (MQ=255)
tACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCa > 1:3273678/1‑53 (MQ=255)
tACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCta > 1:2470676/1‑68 (MQ=255)
aCGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCa > 1:3258471/1‑52 (MQ=255)
aCGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:3670638/70‑1 (MQ=255)
aCGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:3766633/70‑1 (MQ=255)
aCGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:1072016/70‑1 (MQ=255)
cGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAg < 1:3750958/70‑1 (MQ=255)
cGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAg < 1:111760/70‑1 (MQ=255)
cGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAg < 1:1078625/70‑1 (MQ=255)
|
ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAG > W3110S.gb/3442002‑3442135
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
