Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,614,917 |
G→A |
G513G (GGC→GGT) |
ubiB ← |
2‑octaprenylphenol hydroxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,614,917 | 0 | G | A | 100.0%
| 22.9
/ NA
| 9 | G513G (GGC→GGT) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/0); total (9/0) |
TCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCG > W3110S.gb/3614896‑3614960
|
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:105119/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:1299764/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:1339550/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:192505/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:215982/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:569634/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:648150/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:887631/1‑65 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg > 1:890552/1‑65 (MQ=255)
|
TCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCG > W3110S.gb/3614896‑3614960
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
