Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,614,917 |
G→A |
52.1% |
G513G (GGC→GGT) |
ubiB ← |
2‑octaprenylphenol hydroxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,614,917 | 0 | G | A | 52.1%
| ‑1.6
/ 69.8
| 48 | G513G (GGC→GGT) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (9/14); new base A (15/10); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 2.48e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGA > W3110S.gb/3614848‑3614985
|
gATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGc > 1:416213/1‑71 (MQ=255)
cAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc > 1:990997/1‑71 (MQ=255)
cAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCAc > 1:1174636/1‑71 (MQ=255)
ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:205823/71‑1 (MQ=255)
ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:2110476/71‑1 (MQ=255)
ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:1385617/71‑1 (MQ=255)
ccaccTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTaa < 1:231798/71‑1 (MQ=255)
gCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTaa > 1:1673029/1‑71 (MQ=255)
aaCCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTaa > 1:1071810/1‑65 (MQ=255)
ccGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAAt < 1:67486/71‑1 (MQ=255)
cGGGCATCAGCCCCCATTCAGTTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAAt < 1:2686672/70‑1 (MQ=255)
cATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTat < 1:1197271/48‑2 (MQ=255)
tCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGt < 1:188502/55‑1 (MQ=255)
tCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGcc < 1:1684641/61‑1 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTaa > 1:1088292/1‑44 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:1803137/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2725781/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2479351/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:2796303/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:658176/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:68059/1‑70 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa < 1:992663/70‑1 (MQ=255)
aGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGaa > 1:1165927/1‑70 (MQ=255)
ccATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAc > 1:1426278/1‑71 (MQ=255)
cATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTa < 1:502254/43‑1 (MQ=255)
cATTCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcg > 1:2471579/1‑71 (MQ=255)
aTTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc < 1:2199701/71‑1 (MQ=255)
ttCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc > 1:507224/1‑70 (MQ=255)
ttCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgc > 1:1134372/1‑70 (MQ=255)
ttCAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2404954/71‑1 (MQ=255)
cAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1859387/1‑71 (MQ=255)
cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1246856/1‑71 (MQ=255)
cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:281987/1‑71 (MQ=255)
cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1619663/1‑71 (MQ=255)
cAGGTCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:2404255/1‑71 (MQ=255)
gTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaa < 1:1739042/57‑1 (MQ=255)
tCGGCTGACCAACAAGAATGTACCACTTAATACTAACGTAGCGcc < 1:2031058/45‑1 (MQ=255)
gCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGaaa < 1:1380895/54‑1 (MQ=255)
cTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTg < 1:2777513/71‑1 (MQ=255)
caacaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTa > 1:543241/1‑71 (MQ=255)
caacaaGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTa < 1:196981/71‑1 (MQ=255)
aacaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2746013/55‑1 (MQ=255)
acaaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAAcgcg < 1:2778951/54‑1 (MQ=255)
caaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACAt < 1:2412501/71‑1 (MQ=255)
caaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACAt < 1:983359/71‑1 (MQ=255)
aaGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGAt > 1:1616903/1‑54 (MQ=255)
aaGAATGTACCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATg < 1:1100394/71‑1 (MQ=255)
aTGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGAttt < 1:2366453/71‑1 (MQ=255)
tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATaa > 1:2648179/1‑43 (MQ=255)
tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATaa > 1:948471/1‑43 (MQ=255)
tgtaCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTg < 1:768482/71‑1 (MQ=255)
tgCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGa > 1:1101377/1‑70 (MQ=255)
|
GATCAGACCACCTGCCATTAACCAGCCGGGCATCAGCCCCCATTCAGGTCGGCTGACCAACAAGAATGTGCCACTTAATACTAACGTAGCGCCAATTCCGAGAAAATAACGCGATTGTCCCTGACGTACATGATTTGA > W3110S.gb/3614848‑3614985
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
