Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
330,966 |
T→G |
intergenic (+246/‑629) |
betT → / → yahA |
choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 330,966 | 0 | T | G | 97.7%
| 153.2
/ ‑4.3
| 44 | intergenic (+246/‑629) | betT/yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (43/0); total (44/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
CTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA > W3110S.gb/330911‑331022
|
cTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCg > 1:1795397/1‑64 (MQ=255)
aaTGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCaa > 1:1133910/1‑65 (MQ=255)
tgatgaTTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1108299/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAt > 1:1223826/1‑58 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:611412/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2040774/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2067959/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2122769/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:190153/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:2785426/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:291428/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1873807/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:421818/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:46119/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:923304/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1528437/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1277104/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:122507/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1014046/1‑65 (MQ=255)
tGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1037325/1‑65 (MQ=255)
tGATCATTTGAAATGACGCAATATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCa > 1:1419386/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGc > 1:931422/1‑38 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCAtt > 1:81916/1‑45 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:188874/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1044669/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:925090/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1724444/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:801863/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:699810/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1769417/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1769994/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:352826/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2045475/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:281526/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1893948/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2590269/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2430917/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2276446/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2223523/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:193067/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:20930/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2009989/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:2054678/1‑65 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACAGAAATTAACGCAACCa > 1:1774424/1‑43 (MQ=38)
|
CTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTA > W3110S.gb/330911‑331022
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
