Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
330,966 |
T→G |
68.3% |
intergenic (+246/‑629) |
betT → / → yahA |
choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 330,966 | 0 | T | G | 68.3%
| 91.5
/ 24.5
| 44 | intergenic (+246/‑629) | betT/yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (6/8); new base G (13/17); total (19/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 |
AGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTG > W3110S.gb/330903‑331032
|
aGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAggt > 1:2301714/1‑66 (MQ=255)
tttAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCg > 1:756400/1‑70 (MQ=255)
ttAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGt > 1:1764117/1‑70 (MQ=255)
tAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTa < 1:1944454/70‑1 (MQ=255)
aGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaa < 1:2600578/70‑1 (MQ=255)
ttATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATc > 1:1634585/1‑62 (MQ=255)
aTTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAAc > 1:2708560/1‑70 (MQ=255)
aTTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATc < 1:1800089/67‑1 (MQ=255)
tttAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATc < 1:1500617/66‑1 (MQ=255)
tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTaaa > 1:2621362/1‑62 (MQ=255)
tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGa < 1:2399607/70‑1 (MQ=255)
tGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc < 1:1024384/61‑1 (MQ=255)
tGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:717959/71‑1 (MQ=255)
tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc > 1:840056/1‑59 (MQ=255)
tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTa < 1:2504859/70‑1 (MQ=255)
tatTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:1179254/65‑1 (MQ=255)
atTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCaa > 1:2756241/1‑71 (MQ=255)
aTCATTTGAAATGACGCATTATTCATGAGAAAggtg < 1:2746024/36‑1 (MQ=255)
aTCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAAtt < 1:800914/57‑1 (MQ=255)
tCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt < 1:254696/71‑1 (MQ=255)
cATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt > 1:2314381/1‑70 (MQ=255)
cATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCaa > 1:633187/1‑47 (MQ=255)
cATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGaaa > 1:2345187/1‑53 (MQ=255)
tttGAAATGACGCATTATTCATGAGAAATGTGTATCGTa < 1:327490/39‑1 (MQ=255)
aaaTGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTaa < 1:424798/71‑1 (MQ=255)
aaaTGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTaa < 1:408102/71‑1 (MQ=255)
aaTGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg < 1:1691304/71‑1 (MQ=255)
aCGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:605403/71‑1 (MQ=255)
ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGtt < 1:1906227/55‑1 (MQ=255)
ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGtt < 1:2661663/55‑1 (MQ=255)
ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAt < 1:279496/57‑1 (MQ=255)
ttattCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:956653/66‑1 (MQ=255)
attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAt > 1:1339251/1‑55 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt < 1:2410610/52‑1 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGTTTTAATTATc > 1:1906556/1‑71 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTaa > 1:206885/1‑66 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTaa > 1:2074421/1‑66 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:1851106/1‑71 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:475461/1‑71 (MQ=255)
attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:839394/1‑71 (MQ=255)
cATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtg < 1:1846907/70‑1 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtg < 1:2360634/71‑1 (MQ=255)
aTGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtg < 1:808680/71‑1 (MQ=255)
agaAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgt > 1:991285/1‑71 (MQ=255)
agaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgt > 1:456765/1‑71 (MQ=255)
gaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgtg < 1:2620755/71‑1 (MQ=255)
tgtgTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:2657572/52‑1 (MQ=255)
|
AGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTG > W3110S.gb/330903‑331032
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
