Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,894,340 |
C→T |
W32* (TGG→TGA) |
ygcQ ← |
predicted flavoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,894,340 | 0 | C | T | 100.0%
| 149.7
/ NA
| 45 | W32* (TGG→TGA) | ygcQ | predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/19); total (26/19) |
ACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGC > W3110S.gb/2894282‑2894386
|
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCATTg < 1:2281330/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:1050739/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:1123201/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:903389/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:1327984/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:1405966/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:2350635/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:1898756/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:151150/64‑1 (MQ=255)
aCTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg < 1:2836156/64‑1 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTTAGTGCGCCAGGGTAc > 1:1083761/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:486953/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:3101183/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:3056563/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:2909956/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:522093/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:2668914/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:580248/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:853666/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:2300134/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:94087/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:2016166/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:10927/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:1414826/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:1453436/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:1615649/1‑65 (MQ=255)
caACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTAc > 1:1762513/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:272006/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:2864721/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:2869784/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:235144/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:2983646/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:1595566/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:1595422/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:1574718/1‑65 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:1581299/1‑65 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:2282885/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:2006506/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:726077/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:2962762/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:292158/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:1762115/64‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:2733347/64‑1 (MQ=255)
gCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:1184776/63‑1 (MQ=255)
tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCcagccagc < 1:470827/61‑1 (MQ=255)
|
ACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGC > W3110S.gb/2894282‑2894386
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
