Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,894,340 |
C→T |
W32* (TGG→TGA) |
ygcQ ← |
predicted flavoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,894,340 | 0 | C | T | 100.0%
| 178.8
/ NA
| 58 | W32* (TGG→TGA) | ygcQ | predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (48/10); total (48/10) |
CGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC > W3110S.gb/2894275‑2894403
|
cGCTGCCACTGTTTCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg > 1:144263/1‑71 (MQ=255)
cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTTAGGTTCAATCTGTCAGTg > 1:4415330/1‑71 (MQ=255)
cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAg > 1:4023393/1‑69 (MQ=255)
cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg > 1:3278111/1‑71 (MQ=255)
cGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTg > 1:4618330/1‑71 (MQ=255)
ccACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGcc < 1:3639770/70‑1 (MQ=255)
ccACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGcc < 1:323633/70‑1 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAggg > 1:3717808/1‑69 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt > 1:2087681/1‑70 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa > 1:117786/1‑71 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa > 1:3459900/1‑71 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTa > 1:266442/1‑71 (MQ=255)
gTTCCACCAACGCATCCAGCACCTGTTCAGACACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt > 1:1272520/1‑70 (MQ=255)
ttCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt < 1:3602563/69‑1 (MQ=255)
ccaccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACa < 1:2590924/70‑1 (MQ=255)
aaCGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCt > 1:3479621/1‑71 (MQ=255)
cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc > 1:3439755/1‑59 (MQ=255)
cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCt > 1:1652806/1‑71 (MQ=255)
cAGCACCTGTTCCGACACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc > 1:1524413/1‑59 (MQ=255)
cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc > 1:762614/1‑56 (MQ=255)
ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTgcgc > 1:756701/1‑70 (MQ=255)
ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:3562693/1‑71 (MQ=255)
ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:3433486/1‑71 (MQ=255)
ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:3498493/1‑71 (MQ=255)
ccTGTTCCACCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgcc > 1:709480/1‑71 (MQ=255)
cTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccg > 1:3246460/1‑71 (MQ=255)
tGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:4047409/1‑71 (MQ=255)
gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:3590138/1‑70 (MQ=255)
gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:123578/1‑70 (MQ=255)
gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:1301145/1‑70 (MQ=255)
gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:2924053/1‑70 (MQ=255)
gTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc > 1:1526456/1‑70 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc > 1:3248674/1‑48 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAAc > 1:2877263/1‑48 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc > 1:1092643/1‑71 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc > 1:2456754/1‑71 (MQ=255)
ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc > 1:1982810/1‑71 (MQ=255)
aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg > 1:1481412/1‑70 (MQ=255)
aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg > 1:3797129/1‑70 (MQ=255)
aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTg > 1:389639/1‑70 (MQ=255)
aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc > 1:439117/1‑71 (MQ=255)
aCAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc > 1:3859271/1‑71 (MQ=255)
cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc > 1:3479991/1‑70 (MQ=255)
cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc > 1:4274848/1‑70 (MQ=255)
cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCt < 1:1153863/71‑1 (MQ=255)
ggCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTAt < 1:4556896/71‑1 (MQ=255)
tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTg > 1:3296974/1‑70 (MQ=255)
tGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGc > 1:2667706/1‑71 (MQ=255)
gTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATc > 1:1900918/1‑40 (MQ=255)
gTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCa < 1:1960701/71‑1 (MQ=255)
ttCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCAt > 1:965644/1‑71 (MQ=255)
cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTa > 1:2954861/1‑60 (MQ=255)
cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTa > 1:2949499/1‑60 (MQ=255)
cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtc > 1:2767083/1‑71 (MQ=255)
cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtc > 1:1285201/1‑71 (MQ=255)
aaTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCa < 1:3983099/67‑1 (MQ=255)
aTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc < 1:686959/56‑1 (MQ=255)
aTCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc < 1:1711420/56‑1 (MQ=255)
|
CGCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC > W3110S.gb/2894275‑2894403
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
