Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
300,220 |
G→A |
25.0% |
L372L (CTG→CTA) |
mdlB → |
fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 300,220 | 0 | G | A | 25.0%
| 73.2
/ 22.8
| 40 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (6/4); total (19/21) |
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 |
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGG > minE/300150‑300287
|
cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:379275/71‑1 (MQ=255)
tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCg < 1:1536678/71‑1 (MQ=255)
cgcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCgg > 1:1343188/1‑71 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1082135/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg > 1:268813/1‑71 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1224123/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:631516/71‑1 (MQ=255)
gACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:990887/66‑1 (MQ=255)
cAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCg > 1:1319079/1‑71 (MQ=255)
gCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg < 1:1258108/55‑1 (MQ=255)
gCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGc > 1:1168154/1‑70 (MQ=255)
cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCgg < 1:618410/53‑1 (MQ=255)
cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCa > 1:944209/1‑70 (MQ=255)
aaaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:19515/1‑69 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg < 1:2137823/71‑1 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg < 1:1255028/71‑1 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCa > 1:1116827/1‑53 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaa < 1:2163275/69‑1 (MQ=255)
aaCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa > 1:1087537/1‑67 (MQ=255)
aCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc > 1:950404/1‑71 (MQ=255)
aTTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGt < 1:141211/41‑1 (MQ=255)
tAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCg > 1:1445784/1‑71 (MQ=255)
aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAg > 1:1088819/1‑53 (MQ=255)
aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc > 1:1382028/1‑71 (MQ=255)
tctctcTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc < 1:156239/64‑1 (MQ=255)
ctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:1332957/1‑71 (MQ=255)
ctctGCGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAc > 1:1915641/1‑60 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCt > 1:263780/1‑62 (MQ=255)
tgtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:1583097/68‑1 (MQ=255)
tgtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:567435/68‑1 (MQ=255)
tgtgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAt > 1:1078274/1‑71 (MQ=255)
gtgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:979638/67‑1 (MQ=255)
tgCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAtt > 1:1300302/1‑70 (MQ=255)
tgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTGtt < 1:887244/70‑1 (MQ=255)
gCCTTCGAGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1858986/1‑71 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:345946/71‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:287821/71‑1 (MQ=255)
cgcgCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc > 1:1368858/1‑71 (MQ=255)
cAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAtt < 1:1234831/71‑1 (MQ=255)
ttttGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAtt < 1:737735/57‑1 (MQ=255)
gcTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACgg < 1:622717/71‑1 (MQ=255)
|
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGG > minE/300150‑300287
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
