Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
300,220 |
G→A |
100% |
L372L (CTG→CTA) |
mdlB → |
fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 300,220 | 0 | G | A | 90.4%
| 158.2
/ 6.6
| 52 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (0/5); new base A (3/44); total (3/49) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGG > minE/300164‑300270
|
aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt > 1:2139452/1‑66 (MQ=255)
aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt > 1:434404/1‑66 (MQ=255)
aaTCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATa > 1:1308628/1‑67 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGTCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1684018/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:68637/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2098875/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2142873/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:277342/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:370576/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:397087/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:477579/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:526799/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:572088/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1042930/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:687395/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:702602/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:705182/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:751769/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:764243/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:788584/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:793246/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:833328/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:897446/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:992001/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1408668/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1001810/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1066149/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1163889/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1208476/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1244466/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1261087/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1262859/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1317373/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1350313/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1401389/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2093737/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1469723/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1517227/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1524821/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1529337/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1585528/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1590128/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1849661/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1900365/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:1911468/66‑1 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:2008824/66‑1 (MQ=255)
tctcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTa < 1:343729/60‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:621648/67‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:699238/67‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:2116128/67‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:945356/67‑1 (MQ=255)
cAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATggg < 1:976551/66‑1 (MQ=255)
|
AATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGG > minE/300164‑300270
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
