Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
300,220 |
G→A |
51.6% |
L372L (CTG→CTA) |
mdlB → |
fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 300,220 | 0 | G | A | 51.6%
| ‑2.3
/ 97.0
| 64 | L372L (CTG→CTA) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (22/9); new base A (12/21); total (34/30) |
| Fisher's exact test for biased strand distribution p-value = 6.78e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 |
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGC > minE/300150‑300281
|
cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:4209172/71‑1 (MQ=255)
cTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:3760773/71‑1 (MQ=255)
tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:2824477/1‑71 (MQ=255)
tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:2763799/1‑71 (MQ=255)
tCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCg > 1:443999/1‑71 (MQ=255)
cgcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTg < 1:3224950/66‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTTTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2257009/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3003697/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3519378/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2011911/71‑1 (MQ=255)
gcgATGACAATCTGGTGCTAAAGAACATTAATCTCTCTCTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:3251920/71‑1 (MQ=255)
aTGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCAt < 1:2310196/71‑1 (MQ=255)
tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:12521/67‑1 (MQ=255)
tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:111276/67‑1 (MQ=255)
tGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCggg < 1:2141096/67‑1 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:36325/1‑71 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:1718234/1‑71 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:2541250/1‑71 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:768310/1‑71 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAcc > 1:3799045/1‑71 (MQ=255)
aCAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAc > 1:2543159/1‑70 (MQ=255)
gTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCa < 1:4204950/67‑1 (MQ=255)
gTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCa < 1:391510/67‑1 (MQ=255)
tGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGt < 1:2334836/68‑1 (MQ=255)
cTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa < 1:503236/71‑1 (MQ=255)
tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3476498/1‑70 (MQ=255)
tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3245903/1‑70 (MQ=255)
tAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa > 1:3301816/1‑70 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa < 1:3953914/70‑1 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaaa < 1:3197187/70‑1 (MQ=255)
aaGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCaaa < 1:2976/69‑1 (MQ=255)
gAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCa < 1:1089853/71‑1 (MQ=255)
aaCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCAc > 1:3706919/1‑71 (MQ=255)
cATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGATGGTCGGGCATACCGGCAGTGGCAAAAGCAccc < 1:3014939/71‑1 (MQ=255)
aaTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCa > 1:4040436/1‑44 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:3623774/1‑61 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:990464/1‑61 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:2494536/1‑61 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:1802215/1‑61 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc < 1:2904784/70‑1 (MQ=255)
aTCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGc < 1:3571606/70‑1 (MQ=255)
aTCTCTCAGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAg > 1:2367394/1‑61 (MQ=255)
tctctGTGCCTTCGCGCAATTTTTTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:1412274/1‑71 (MQ=255)
tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:727537/1‑71 (MQ=255)
tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:721540/1‑71 (MQ=255)
tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:2038429/1‑71 (MQ=255)
tctctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt > 1:2177623/1‑71 (MQ=255)
tctctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCAcc > 1:157531/1‑62 (MQ=255)
ctctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:546581/1‑71 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAg < 1:1658079/68‑1 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAg < 1:531431/68‑1 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:565022/1‑70 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:2409741/1‑70 (MQ=255)
tctGTGCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt > 1:1399864/1‑70 (MQ=255)
tgtgCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt < 1:666824/68‑1 (MQ=255)
gCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1056925/1‑71 (MQ=255)
gCCTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGa > 1:1252058/1‑71 (MQ=255)
ccTTCGCGCAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt < 1:1731823/71‑1 (MQ=255)
cgcgCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc > 1:1107190/1‑71 (MQ=255)
gcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc < 1:651575/70‑1 (MQ=255)
gcgcAATTTTGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGc < 1:2844454/70‑1 (MQ=255)
cgcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCt < 1:1662521/70‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAt < 1:1195153/71‑1 (MQ=255)
gcAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAt < 1:406235/71‑1 (MQ=255)
aaTTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTa < 1:1468447/71‑1 (MQ=255)
ttGTGGCGCTAGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCg > 1:1720423/1‑71 (MQ=255)
gtgGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGc > 1:1221776/1‑70 (MQ=255)
|
CTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGC > minE/300150‑300281
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
