Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,910,539 |
C→A |
R590L (CGC→CTC) |
relA ← |
(p)ppGpp synthetase I/GTP pyrophosphokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,910,539 | 0 | C | A | 100.0%
| 79.4
/ NA
| 25 | R590L (CGC→CTC) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (3/22); total (3/22) |
AGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGC > W3110S.gb/2910471‑2910595
|
agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg < 1:734652/71‑1 (MQ=255)
agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg < 1:3447665/71‑1 (MQ=255)
agcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAgg < 1:2241646/71‑1 (MQ=255)
gcagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGt < 1:3838162/71‑1 (MQ=255)
cagcGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGt < 1:747917/70‑1 (MQ=255)
cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc < 1:175077/71‑1 (MQ=255)
cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc < 1:3321728/71‑1 (MQ=255)
cgATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcggc < 1:2783797/71‑1 (MQ=255)
gtgCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTa > 1:335928/1‑69 (MQ=255)
gtgCATCAGGTTGCCAACACATTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGc > 1:2142549/1‑71 (MQ=255)
acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTTCGGCGTGTAGCTTTTTTGCTGAAGt < 1:43733/69‑1 (MQ=255)
acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGACGt < 1:1766391/69‑1 (MQ=255)
acacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGt < 1:1444257/69‑1 (MQ=255)
cacCTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTgcgga < 1:403761/47‑2 (MQ=255)
ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg < 1:3073187/68‑1 (MQ=255)
ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg < 1:621669/68‑1 (MQ=255)
ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg < 1:2197758/68‑1 (MQ=255)
ccTTCGACTACCACGCGACCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTg < 1:1939513/68‑1 (MQ=255)
aCCGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTT‑CTTCAGCGCGGCGGCGTCCTg < 1:4066058/71‑1 (MQ=255)
ccGTTATCTTTACTTAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg < 1:1964488/71‑1 (MQ=255)
ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg < 1:410496/71‑1 (MQ=255)
ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg < 1:199681/71‑1 (MQ=255)
ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg < 1:2628262/71‑1 (MQ=255)
ccGTTATCTTTACTGAGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTg < 1:2462666/71‑1 (MQ=255)
tACTGAGGTTTTGCGGCGTGTAGCTTTTTTTCTGAAGTTGCTTCAGCGCGGCGGCGTCCAGc > 1:312100/1‑62 (MQ=255)
|
AGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGC > W3110S.gb/2910471‑2910595
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
