Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
668,428 |
C→A |
T589K (ACG→AAG) |
uup → |
fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 668,428 | 0 | C | A | 100.0%
| 112.0
/ NA
| 38 | T589K (ACG→AAG) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/16); total (22/16) |
CAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATA > minE/668361‑668493
|
cAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGc < 1:2113193/70‑1 (MQ=255)
cAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGc < 1:2038486/70‑1 (MQ=255)
aGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCaa < 1:110991/71‑1 (MQ=255)
gcgcGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAATCCCTACAGAAGCAAg < 1:1074870/71‑1 (MQ=255)
cgcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGt > 1:2454192/1‑71 (MQ=255)
agcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGt > 1:1072690/2‑71 (MQ=255)
gcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTg < 1:995853/71‑1 (MQ=255)
gcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTg < 1:2529374/71‑1 (MQ=255)
gAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGc < 1:2056196/71‑1 (MQ=255)
gcagcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGct > 1:1649156/1‑70 (MQ=255)
ccGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCcttctt < 1:1037864/71‑1 (MQ=255)
ccGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCcttctt > 1:881389/1‑71 (MQ=255)
ccGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCcttct > 1:1956934/1‑70 (MQ=255)
ccGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCcttct < 1:376697/70‑1 (MQ=255)
ccGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCcttct < 1:2185933/70‑1 (MQ=255)
gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcc > 1:256711/1‑58 (MQ=255)
gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcc > 1:2347050/1‑58 (MQ=255)
gCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGc > 1:2910328/1‑71 (MQ=255)
gAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttc > 1:1120005/1‑53 (MQ=255)
aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt > 1:1189476/1‑70 (MQ=255)
tCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGc > 1:2773456/1‑62 (MQ=255)
gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAg < 1:611141/69‑1 (MQ=255)
gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAg < 1:2084607/69‑1 (MQ=255)
gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa < 1:2589485/71‑1 (MQ=255)
aGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaa > 1:1008245/1‑69 (MQ=255)
ggAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgc > 1:351165/1‑71 (MQ=255)
gCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACTCAAAAAGtgcttgc < 1:2073463/71‑1 (MQ=255)
tACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGa > 1:1481588/1‑70 (MQ=255)
tACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGa > 1:1870227/1‑70 (MQ=255)
tACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGa > 1:277765/1‑70 (MQ=255)
aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCa > 1:2676054/1‑42 (MQ=255)
aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt > 1:2172822/1‑43 (MQ=255)
aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg > 1:2117841/1‑59 (MQ=255)
cAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgct > 1:1707107/1‑62 (MQ=255)
cAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata < 1:156143/70‑1 (MQ=255)
aGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCg < 1:1858113/38‑1 (MQ=255)
aGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaaaaa > 1:2051165/1‑56 (MQ=255)
aGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg > 1:998300/1‑63 (MQ=255)
|
CAGCGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATA > minE/668361‑668493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
