Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 668,428 C→A T589K (ACG→AAG)  uup → fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE668,4280CA100.0% 135.4 / NA 43T589K (ACG→AAG) uupfused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/12);  total (31/12)

GCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTG  >  minE/668378‑668487
                                                  |                                                           
gcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcct                                         <  1:3589492/71‑1 (MQ=255)
gcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcct                                         <  1:4351163/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:4112299/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:4000990/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:1855628/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:2038298/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:3630971/71‑1 (MQ=255)
     cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:3693028/71‑1 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCGGCCGCAt                        >  1:3973518/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3917225/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3430297/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3418618/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:312790/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2976583/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2406268/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2328965/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1397024/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1299029/1‑70 (MQ=255)
                  aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1190981/1‑70 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2782553/1‑66 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3835578/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3638154/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:834656/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3999906/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:4322515/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:2673706/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:2858722/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:1285282/1‑69 (MQ=255)
                      cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTAAGTCAGCCGCATGAg                     >  1:379599/1‑69 (MQ=255)
                         gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:3830334/70‑1 (MQ=255)
                         gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:567872/70‑1 (MQ=255)
                         gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:2199908/70‑1 (MQ=255)
                         gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:3987675/70‑1 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1214085/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:682886/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:490764/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1380406/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1846082/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:2283530/1‑70 (MQ=255)
                              gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:2437801/1‑70 (MQ=255)
                                       aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg  >  1:1786893/1‑71 (MQ=255)
                                       aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg  >  1:2453220/1‑71 (MQ=255)
                                       aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgctt   >  1:2113190/1‑70 (MQ=255)
                                                  |                                                           
GCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTG  >  minE/668378‑668487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A