Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,811,850 |
G→A |
intergenic (+88/‑75) |
yniC → / → ydjM |
predicted hydrolase/predicted inner membrane protein regulated by LexA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,811,850 | 0 | G | A | 97.4%
| 111.1
/ ‑3.8
| 39 | intergenic (+88/‑75) | yniC/ydjM | predicted hydrolase/predicted inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/16); total (22/17) |
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTA > W3110S.gb/1811784‑1811912
|
cTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac < 1:2659988/71‑1 (MQ=255)
ccTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGt > 1:2784983/1‑71 (MQ=255)
ccTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGt < 1:224465/71‑1 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:794314/1‑71 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTca > 1:4518059/1‑70 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTca > 1:2840254/1‑70 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTc > 1:3780165/1‑69 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac < 1:3310134/70‑1 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:4586793/1‑70 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcac > 1:3432059/1‑70 (MQ=255)
tCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTa > 1:4048332/1‑59 (MQ=255)
tCTTTATCTGAAATTGCATT‑AACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTa > 1:4070978/1‑58 (MQ=255)
cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACt > 1:2706661/1‑71 (MQ=255)
tATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTcc > 1:4110264/1‑71 (MQ=255)
tGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGTAAGAATTACACCGTCACCACTTCCGTg > 1:755018/1‑70 (MQ=255)
aaaTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa < 1:2391587/70‑1 (MQ=255)
aaaTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTg < 1:2020062/68‑1 (MQ=255)
aTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTg > 1:1184802/1‑71 (MQ=255)
aTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTg > 1:3207239/1‑71 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:3152338/1‑70 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:1956926/1‑70 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGta > 1:1910147/1‑70 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTaca > 1:1282634/1‑41 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTaca > 1:4677600/1‑41 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATaaaaa > 1:80453/1‑71 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATaaaaa > 1:4347940/1‑71 (MQ=255)
cTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc < 1:3710219/71‑1 (MQ=255)
cTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc < 1:4592814/71‑1 (MQ=255)
aCGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:3991247/70‑1 (MQ=255)
cGGATTAATCGTCAATTTAAGAGTAAGAATTACTCCGTCTCCACTTCCGTGc < 1:1968171/52‑1 (MQ=255)
ggATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc < 1:2775090/71‑1 (MQ=255)
aTTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTg < 1:1166711/71‑1 (MQ=255)
tCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:3495182/61‑1 (MQ=255)
gTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTAt > 1:2842856/1‑71 (MQ=255)
agagAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:2942187/71‑1 (MQ=255)
agagAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:2863066/71‑1 (MQ=255)
agagAAAGAATTACACCGTCACCACTTCCGTCCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:641564/71‑1 (MQ=255)
gagaAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa < 1:3884860/71‑1 (MQ=255)
aaaGAATTACACCGTCACCACTTCCGTGCACTGTATaaa < 1:2075538/39‑1 (MQ=255)
agaaTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa > 1:2277466/1‑51 (MQ=255)
|
CTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTA > W3110S.gb/1811784‑1811912
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
