Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,811,850 |
G→A |
32.3% |
intergenic (+88/‑75) |
yniC → / → ydjM |
predicted hydrolase/predicted inner membrane protein regulated by LexA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,811,850 | 0 | G | A | 32.3%
| 80.7
/ 52.8
| 62 | intergenic (+88/‑75) | yniC/ydjM | predicted hydrolase/predicted inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base G (25/17); new base A (11/9); total (36/26) |
| Fisher's exact test for biased strand distribution p-value = 7.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 |
GCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTG > W3110S.gb/1811786‑1811915
|
gCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTac < 1:1749628/69‑1 (MQ=255)
gCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTac < 1:328122/69‑1 (MQ=255)
gCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac < 1:3439085/69‑1 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCTTCAATTTAAGAGAAAGAGTTACACCGt > 1:1923650/1‑68 (MQ=255)
ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTc > 1:1715366/1‑69 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt < 1:3427366/59‑1 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt < 1:253774/59‑1 (MQ=255)
gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc > 1:35234/1‑68 (MQ=255)
cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca > 1:2112553/1‑69 (MQ=255)
ttATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt > 1:1256121/1‑54 (MQ=255)
tATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACtt > 1:2682109/1‑69 (MQ=255)
tATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACtt > 1:3586032/1‑69 (MQ=255)
cTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg > 1:1521324/1‑69 (MQ=255)
cTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac > 1:1536973/1‑53 (MQ=255)
cTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac > 1:387906/1‑53 (MQ=255)
cTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac > 1:1736416/1‑53 (MQ=255)
cTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTac > 1:372956/1‑53 (MQ=255)
tGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg < 1:3633688/68‑1 (MQ=255)
tGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg < 1:2018673/68‑1 (MQ=255)
tGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCg < 1:3191026/68‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcac > 1:2410016/1‑53 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcac > 1:1457882/1‑53 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcac > 1:1224210/1‑53 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:587590/54‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:802214/54‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:954184/54‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:958506/54‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:1552121/54‑1 (MQ=255)
gCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTcacc < 1:2113484/54‑1 (MQ=255)
ttCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt > 1:2486345/1‑39 (MQ=255)
tCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGtata > 1:2147404/1‑68 (MQ=255)
cAACTGACGGATTAATCGTCATTTTAAGAGAAagaga > 1:2666313/1‑36 (MQ=39)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt > 1:1232786/1‑37 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt > 1:3604430/1‑37 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt < 1:3521411/37‑1 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa < 1:2691244/69‑1 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATaaa > 1:2067343/1‑69 (MQ=255)
cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAATTACACCGTCACCACTTCCATGCACTGTATaaa > 1:434279/1‑69 (MQ=255)
aCTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaa < 1:3518925/68‑1 (MQ=255)
tGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCATCACTTCCGTGCACTGTATAAAAAt > 1:1448097/1‑68 (MQ=255)
tGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAAt > 1:1803073/1‑68 (MQ=255)
tGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAAt > 1:1291673/1‑68 (MQ=255)
ggATTAATCGTCAATTTAAGAGAAAGAGTTACACCGt < 1:416526/37‑1 (MQ=255)
ggATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:2021152/68‑1 (MQ=255)
ttAATCGTCAATTTAAGATAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc > 1:1839285/1‑68 (MQ=255)
ttAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc > 1:3474402/1‑68 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc > 1:2210567/1‑61 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc > 1:2545785/1‑61 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:2910959/63‑1 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:84235/63‑1 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta > 1:3280414/1‑63 (MQ=255)
aaTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:2823677/63‑1 (MQ=255)
aTCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg > 1:2559666/1‑44 (MQ=255)
gTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGt < 1:807510/69‑1 (MQ=255)
cAATTTAAGAGAAAGAATTACACCGTCACCACTTcc > 1:3350237/1‑36 (MQ=255)
aaTTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta < 1:809216/56‑1 (MQ=255)
tttAAGAGAAAGAATTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGa < 1:241861/69‑1 (MQ=255)
tAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACa > 1:1896950/1‑69 (MQ=255)
tAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACa > 1:1498511/1‑69 (MQ=255)
tAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACa > 1:3398175/1‑69 (MQ=255)
agaaagaaTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt < 1:899604/69‑1 (MQ=255)
agaAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGtt < 1:3567748/68‑1 (MQ=255)
aaaGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa > 1:3565724/1‑68 (MQ=255)
aaaGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa > 1:3532317/1‑68 (MQ=255)
agagTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg > 1:2073224/1‑69 (MQ=255)
agagTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg > 1:214404/1‑69 (MQ=255)
|
GCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTG > W3110S.gb/1811786‑1811915
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
