Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R2 328 63.0 4687567 98.0% 4593815 65.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,285,913 T→C R21R (CGT→CGC)  rplY → 50S ribosomal subunit protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,285,9130TC100.0% 81.1 / NA 26R21R (CGT→CGC) rplY50S ribosomal subunit protein L25
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

GTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGC  >  W3110S.gb/2285853‑2285974
                                                            |                                                             
gTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAAc                                                      >  1:556107/1‑70 (MQ=255)
 tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTaa                                                       >  1:2381852/1‑68 (MQ=255)
 tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAAc                                                      >  1:2960155/1‑69 (MQ=255)
 tttACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACa                                                     <  1:3727966/70‑1 (MQ=255)
   tACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAg                                                   <  1:1893742/70‑1 (MQ=255)
     cTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGtt                                                 <  1:4456418/70‑1 (MQ=255)
        tCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTc                                                >  1:649938/1‑68 (MQ=255)
        tCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCg                                             >  1:551694/1‑71 (MQ=255)
          aaCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCgg                                            >  1:4121241/1‑70 (MQ=255)
           aCGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCa                                          <  1:2079575/71‑1 (MQ=255)
              cAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatc                                       <  1:743812/71‑1 (MQ=255)
               aGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatca                                      <  1:2707760/71‑1 (MQ=255)
                   gTACGTAAAGAGCAGGGTAAGGTTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTa                                  <  1:997250/71‑1 (MQ=255)
                   gTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTa                                  >  1:539427/1‑71 (MQ=255)
                    tACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:3754485/71‑1 (MQ=255)
                     aCGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACg                                >  1:2203292/1‑71 (MQ=255)
                          aaGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCa                                          <  1:2110143/56‑1 (MQ=255)
                          aaGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTgg                            >  1:2569274/1‑70 (MQ=255)
                               cAGGGTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAatca                                      <  1:3211188/55‑1 (MQ=255)
                                   gTAAGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAgcg                  >  1:1785656/1‑71 (MQ=255)
                                      aGGGTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGcgccg               >  1:1058780/1‑71 (MQ=255)
                                         gTGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:1508047/50‑1 (MQ=255)
                                          tGCGAGCCGCCGCCTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTAc                                 <  1:3981145/49‑1 (MQ=255)
                                                 cgccgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCg     <  1:1001972/70‑1 (MQ=255)
                                                  gccgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAg   >  1:3731114/1‑71 (MQ=255)
                                                    cgccTGCGCGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGc  <  1:219536/70‑1 (MQ=255)
                                                            |                                                             
GTTTACTATCAACGCAGAAGTACGTAAAGAGCAGGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCCGGCAATCATCTACGGTGGCAAAGAAGCGCCGCTGGCTATCGAGC  >  W3110S.gb/2285853‑2285974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A