Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,536,834 C→T M1I (ATG→ATA) † yfcR ← predicted fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,536,8340CT100.0% 272.2 / NA 78M1I (ATG→ATA) †yfcRpredicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (57/21);  total (57/21)

AGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGG  >  minE/1536773‑1536890
                                                             |                                                        
aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:4262826/71‑1 (MQ=255)
aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:2467882/71‑1 (MQ=255)
aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:2021872/71‑1 (MQ=255)
aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:1781376/71‑1 (MQ=255)
aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:1421581/71‑1 (MQ=255)
     cTGCATACCAGCAGCCCTGCTCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:437341/71‑1 (MQ=255)
     cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:3774428/71‑1 (MQ=255)
     cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:588126/71‑1 (MQ=255)
     cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:1463530/71‑1 (MQ=255)
     cTGCATACCAGCAGCCCTGCGCACACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:2005668/71‑1 (MQ=255)
          tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:3690818/1‑71 (MQ=255)
          tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:3296299/1‑71 (MQ=255)
          tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:2389325/1‑71 (MQ=255)
          tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:1907991/1‑71 (MQ=255)
                    ccTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATc                              >  1:1349421/1‑70 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1629466/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:544981/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:3906231/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1146080/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:4290291/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:2042834/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:193365/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:3499219/51‑1 (MQ=255)
                              cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1428853/51‑1 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3165259/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3197437/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3647744/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3365645/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:568456/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:556635/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3471795/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:2587051/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3874856/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:2186266/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:4038052/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1963784/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:195026/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1927049/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1701896/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1669606/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1077697/1‑71 (MQ=255)
                                aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGACTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:405805/1‑71 (MQ=255)
                                   aaCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            <  1:3677892/57‑1 (MQ=255)
                                   aaCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            <  1:4077832/57‑1 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCAt                               >  1:3603770/1‑52 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGc                      >  1:890611/1‑61 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGcag                 >  1:399355/1‑64 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:571499/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:4238809/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:1388477/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:1684090/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2080208/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:758939/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2306531/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:478202/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2950050/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:3048456/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:575648/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:3491078/1‑70 (MQ=255)
                                     cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCGCTTCATCCACCTGGCAGCTGTTGc             >  1:2907343/1‑70 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            >  1:594585/1‑53 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:745199/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:772441/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3221485/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:4206002/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3410880/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3340864/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3293772/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3066389/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:2107712/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:1181021/1‑54 (MQ=255)
                                       tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGCCTCTGCTCTTCATCCAc                           >  1:1063981/1‑54 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCAt                               >  1:3055291/1‑40 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCAtt         >  1:3985678/1‑62 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:3805312/1‑69 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:1056798/1‑69 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:2639003/1‑69 (MQ=255)
                                                 aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:2580314/1‑69 (MQ=255)
                                                             |                                                        
AGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGG  >  minE/1536773‑1536890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A