Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,777,713 T→C N105D (AAT→GAT)  trmD ← tRNA (guanine‑1‑)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,777,7130TC100.0% 405.0 / NA 107N105D (AAT→GAT) trmDtRNA (guanine‑1‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/83);  total (24/83)

Only 100 of 107 total aligned reads displayed.

ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773
                                                       |                                                            
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:898234/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1858624/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2041318/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2080650/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1760662/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2085258/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1700298/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:28105/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3310686/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3529932/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1409267/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3863921/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3635530/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:457011/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1180963/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1330343/71‑1 (MQ=255)
aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:4028495/71‑1 (MQ=255)
 tCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1400040/70‑1 (MQ=255)
                  ccTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:4272235/53‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2694513/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2225840/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2344355/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3927871/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2474816/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2658076/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3023921/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3037784/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3138691/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1782238/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1421702/69‑1 (MQ=255)
                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1228622/69‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:868296/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:3156119/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1628759/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:276014/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:110947/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:2674609/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:256835/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1663208/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1400094/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:239776/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:401044/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1187419/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:646706/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:660364/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:672101/59‑1 (MQ=255)
                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCACGCTTGATCAAg                             <  1:1518466/59‑1 (MQ=255)
                              ccGCACACCACAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1551345/59‑1 (MQ=255)
                               cGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:3476892/58‑1 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:3666287/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:4107/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1143526/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:636982/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:3422433/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:712474/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1122794/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:295411/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:192687/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:161891/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:2783312/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:209410/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1651489/1‑59 (MQ=255)
                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:252869/1‑59 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3762359/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:2273857/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1371703/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:2272240/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1200738/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:52682/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:596239/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1803408/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:847399/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1817239/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3155101/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:291648/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3513140/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3491250/71‑1 (MQ=255)
                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3379874/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3727918/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3127976/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1961501/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2011368/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:609093/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3280330/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1752509/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:4282727/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:4123528/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1731406/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2497518/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2433887/71‑1 (MQ=255)
                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3732774/71‑1 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            >  1:3220197/1‑69 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:402399/1‑70 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:635729/1‑70 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:2048195/1‑70 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1728457/1‑70 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1899962/1‑70 (MQ=255)
                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1588094/1‑70 (MQ=255)
                                        gAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3516269/51‑1 (MQ=255)
                                               tttttGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:623848/69‑1 (MQ=255)
                                                       |                                                            
ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A