Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R1
|
122 |
92.7 |
4382561 |
94.3% |
4132755 |
66.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,450,298 |
A→G |
W78R (TGG→CGG) |
yifE ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,450,298 | 0 | A | G | 99.6%
| 884.3
/ ‑4.9
| 228 | W78R (TGG→CGG) | yifE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/117); minor base T (1/0); total (111/117) |
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 |
Only 100 of 251 total aligned reads displayed.
CTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC > minE/2450230‑2450364
|
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:2151364/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:2430492/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:4291582/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:4199564/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:3079328/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:3955183/1‑71 (MQ=255)
cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:328879/1‑71 (MQ=255)
tGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCgcac > 1:933082/1‑71 (MQ=255)
tGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCgcac > 1:2603854/1‑71 (MQ=255)
cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:19403/70‑1 (MQ=255)
cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:772259/70‑1 (MQ=255)
cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:874553/70‑1 (MQ=255)
cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGCACCGCAcg < 1:2581289/70‑1 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:3727607/1‑71 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt < 1:4033884/71‑1 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:1001411/1‑71 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:685579/1‑71 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:2017060/1‑71 (MQ=255)
gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt < 1:2974384/71‑1 (MQ=255)
gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGt < 1:2495455/71‑1 (MQ=255)
gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGt < 1:2339550/71‑1 (MQ=255)
aaGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCa > 1:1714021/1‑71 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2996790/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1765232/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2010597/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2501936/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:3097402/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:3225157/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2781747/71‑1 (MQ=255)
ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1422028/71‑1 (MQ=255)
gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:1097202/1‑71 (MQ=255)
gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:3624260/1‑71 (MQ=255)
gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:4307584/1‑71 (MQ=255)
gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg > 1:208078/1‑70 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCt > 1:3548957/1‑59 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2363859/1‑71 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:1200830/1‑71 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2686990/1‑71 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2678202/1‑71 (MQ=255)
tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:819774/1‑71 (MQ=255)
tgAAAACGTTTTGGACGCTTAATTCGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1566325/67‑1 (MQ=255)
aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:2461266/1‑71 (MQ=255)
aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:703993/1‑71 (MQ=255)
aaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGt < 1:2280128/71‑1 (MQ=255)
ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:3669814/71‑1 (MQ=255)
ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:4271415/71‑1 (MQ=255)
ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:2862687/71‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3322970/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1801589/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3804050/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3283371/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1036360/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:2544005/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1604015/70‑1 (MQ=255)
ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:2313498/70‑1 (MQ=255)
cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:1579005/71‑1 (MQ=255)
cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:3655408/71‑1 (MQ=255)
cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:2229099/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3026347/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3904819/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:4176663/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3370793/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:2390805/71‑1 (MQ=255)
gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:1168366/71‑1 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACt > 1:3682075/1‑36 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:1342004/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:2292546/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3754666/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:1195741/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3059962/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:2631125/1‑58 (MQ=255)
atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3422933/1‑58 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc < 1:2816387/44‑1 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc < 1:409239/44‑1 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:1497118/1‑71 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:2651355/1‑71 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:3935818/1‑70 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:4022947/1‑70 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:2721568/1‑70 (MQ=255)
taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:3492520/1‑70 (MQ=255)
taCTTGGACCGCAAGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:1744438/1‑71 (MQ=255)
cTTGGACCTCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:1695849/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGtt > 1:1466697/1‑41 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCg > 1:854968/1‑43 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:354046/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:3216240/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:523819/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:4349745/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:1511125/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:3827752/1‑44 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtct > 1:2527000/1‑71 (MQ=255)
cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtct > 1:4142643/1‑71 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:3160654/69‑1 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:16246/69‑1 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:2059404/69‑1 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:386739/69‑1 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:4084459/69‑1 (MQ=255)
ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct < 1:1903656/70‑1 (MQ=255)
gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc < 1:4200813/71‑1 (MQ=255)
gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc < 1:568620/71‑1 (MQ=255)
|
CTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC > minE/2450230‑2450364
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
