Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
686,526 |
A→G |
I189T (ATC→ACC) |
gltJ ← |
glutamate and aspartate transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 686,526 | 0 | A | G | 100.0%
| 98.1
/ NA
| 27 | I189T (ATC→ACC) | gltJ | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/11); total (16/11) |
CATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC > W3110S.gb/686470‑686582
|
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2077222/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:589279/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:3038509/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2809812/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:619510/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2547976/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2486356/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2450567/65‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:1696677/65‑1 (MQ=255)
aTGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:1715168/64‑1 (MQ=255)
aTGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAg < 1:2848223/64‑1 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAg > 1:1332897/1‑40 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTcatcat > 1:1485399/1‑49 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt > 1:751767/1‑61 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:703271/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:896539/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:624825/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:797408/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:1210566/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:526534/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:407001/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:2724329/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:2280821/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:1929798/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:146044/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:1409953/1‑65 (MQ=255)
ccaGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggc > 1:1301527/1‑65 (MQ=255)
|
CATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGC > W3110S.gb/686470‑686582
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
