Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
686,526 |
A→G |
9.6% |
I189T (ATC→ACC) |
gltJ ← |
glutamate and aspartate transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 686,526 | 0 | A | G | 9.6%
| 109.9
/ 3.3
| 43 | I189T (ATC→ACC) | gltJ | glutamate and aspartate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (13/25); new base G (2/2); total (15/28) |
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCG > W3110S.gb/686457‑686596
|
aaCGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGa > 1:3098434/1‑70 (MQ=255)
cGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAg > 1:1087379/1‑71 (MQ=255)
cGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAg > 1:728130/1‑71 (MQ=255)
cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGc < 1:3578638/71‑1 (MQ=255)
cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGGTAGTCGAGGc < 1:2413553/71‑1 (MQ=255)
cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGc < 1:915338/71‑1 (MQ=255)
cAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGc < 1:1431719/71‑1 (MQ=255)
aGGCATGGGCTGAGTAATCTAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCg < 1:3774345/71‑1 (MQ=255)
ggCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGTTAGTCGAGGc < 1:1624740/69‑1 (MQ=255)
gCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAgg < 1:1222474/67‑1 (MQ=255)
cATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCg < 1:2305470/68‑1 (MQ=255)
ggCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCg < 1:3423506/70‑1 (MQ=255)
ggCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCg > 1:3085275/1‑70 (MQ=255)
gTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGCCGGAGtttt > 1:61130/1‑71 (MQ=255)
tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat < 1:2035903/70‑1 (MQ=255)
tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat > 1:13223/1‑70 (MQ=255)
tACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcat > 1:3209850/1‑70 (MQ=255)
aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:598773/70‑1 (MQ=255)
aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:1970591/70‑1 (MQ=255)
aCCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3341187/70‑1 (MQ=255)
tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3724307/63‑1 (MQ=255)
tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2434993/63‑1 (MQ=255)
tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:34082/63‑1 (MQ=255)
tGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:3151563/63‑1 (MQ=255)
gccATATCCACCAGACCGGTAGTAGAGGCGATGGCGGAGttt > 1:1048803/1‑42 (MQ=38)
gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGt > 1:2008467/1‑40 (MQ=255)
gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:2757466/1‑54 (MQ=255)
gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt > 1:3224135/1‑71 (MQ=255)
gccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCAt > 1:3873259/1‑71 (MQ=255)
ccATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATc < 1:2488558/71‑1 (MQ=255)
tatCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCAcc < 1:1703542/44‑1 (MQ=255)
tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtca > 1:2130216/1‑49 (MQ=255)
tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:1834182/1‑48 (MQ=255)
tCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATcggcgg > 1:2125563/1‑71 (MQ=255)
ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTc < 1:2787015/35‑1 (MQ=255)
ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2488087/47‑1 (MQ=255)
ccaGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtcatc < 1:2861330/47‑1 (MQ=255)
caGACCGGTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtc > 1:3038552/1‑43 (MQ=255)
aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:357717/71‑1 (MQ=255)
aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:272431/71‑1 (MQ=255)
aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:573374/71‑1 (MQ=255)
aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:1934036/71‑1 (MQ=255)
aGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGAt < 1:715709/71‑1 (MQ=255)
gACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTtca < 1:466304/42‑1 (MQ=255)
aTAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAAcgcg < 1:3459623/71‑1 (MQ=255)
|
AACGACTCCCAGGCATGGGCTGAGTAATCGAGCAATTTACCCGCCTGCGCCGCCATATCCACCAGACCGATAGTCGAGGCGATGGCGGAGTTTTTCACCAGGTTCATCATCTCTGAGGTCATCGGCGGGACGATAACGCG > W3110S.gb/686457‑686596
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
