Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F17 I0 R2	278	43.2	2215644	94.6%	2095999	61.4

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,027,446	A→G	30.8%	N134S (AAC→AGC)	malX →	fused maltose and glucose‑specific PTS enzyme IIBC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,027,446	0	A	G	30.8%	30.2 / 18.6	26	N134S (AAC→AGC)	malX	fused maltose and glucose‑specific PTS enzyme IIBC components
Reads supporting (aligned to +/- strand): ref base A (9/9); new base G (4/4); total (13/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATC  >  minE/1027376‑1027489

gcgGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATaa                                             >  1:1738975/1‑71 (MQ=255)

       aCTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCag                                      <  1:209211/71‑1 (MQ=255)

          tCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag                                    >  1:1052178/1‑70 (MQ=255)

           cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa                                  >  1:287880/1‑71 (MQ=255)

           cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCagag                                    <  1:2061924/69‑1 (MQ=255)

                 gACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCtt                             <  1:1731545/70‑1 (MQ=255)

                      aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc                       >  1:602019/1‑71 (MQ=255)

                      aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc                       <  1:1760587/71‑1 (MQ=255)

                      aTAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc                       <  1:737154/71‑1 (MQ=255)

                       tAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATc                       <  1:718484/70‑1 (MQ=255)

                         aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCa                     <  1:1506200/70‑1 (MQ=255)

                         aaGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCa                     <  1:746430/70‑1 (MQ=255)

                            gCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcg                 <  1:1962751/71‑1 (MQ=255)

                               ttCTGCCAACCACGGATTCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCa                     <  1:1882941/64‑1 (MQ=255)

                                 cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga            >  1:1841893/1‑71 (MQ=255)

                                 cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcga            >  1:1485260/1‑71 (MQ=255)

                                 cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgatcg             >  1:473532/1‑70 (MQ=255)

                                 cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcg                 <  1:1272354/66‑1 (MQ=255)

                                         cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATa          >  1:1052549/1‑65 (MQ=255)

                                         cACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGa    >  1:131600/1‑71 (MQ=255)

                                          aCGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGtcgat               >  1:1739958/1‑59 (MQ=255)

                                           cGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATc  <  1:805618/71‑1 (MQ=255)

                                                 ccGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCt                              >  1:1890070/1‑37 (MQ=255)

                                                       ttCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATc                       <  1:1418467/38‑1 (MQ=255)

                                                           gAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcta            >  1:194858/1‑43 (MQ=255)

                                                           gAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga            >  1:637755/1‑45 (MQ=255)

                                                                  aaTAACATCCAGAGCATTCTTTGGATCCAGTCGATCGATAc         >  1:344359/1‑41 (MQ=255)

                                                                     aaCATCCAGAGCATTCTTGGGATCCAGtcgatcga            >  1:797074/1‑35 (MQ=255)

GCGGTAAACTTCTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATC  >  minE/1027376‑1027489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A