Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I2 R1 28 4.9 280516 80.6% 226095 64.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,027,446 A→G N134S (AAC→AGC)  malX → fused maltose and glucose‑specific PTS enzyme IIBC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,027,4460AG100.0% 19.2 / NA 7N134S (AAC→AGC) malXfused maltose and glucose‑specific PTS enzyme IIBC components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/2);  total (5/2)

CTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGT  >  minE/1027387‑1027510
                                                           |                                                                
cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa                                                       >  1:18368/1‑71 (MQ=255)
cTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCa                                                       >  1:52216/1‑71 (MQ=255)
                      cTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga                                 >  1:202137/1‑71 (MQ=255)
                              cACGGATGCCGCGGTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGtcgatcga                                 >  1:196762/1‑63 (MQ=255)
                                           gTTCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggt            >  1:267591/1‑71 (MQ=255)
                                            ttCTGAAAGCCAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTcggtgcggtg           <  1:256129/71‑1 (MQ=255)
                                                     ccAATAGCATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGt  <  1:89948/71‑1 (MQ=255)
                                                           |                                                                
CTGGTTGACCAATAAAGGCATTCTGCCAACCACGGATGCCGCGGTTCTGAAAGCCAATAACATCCAGAGCATTCTTGGGATCCAGTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGT  >  minE/1027387‑1027510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A