Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,531,609 |
A→G |
7.3% |
I14I (ATT→ATC) |
coaD ← |
pantetheine‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,531,609 | 0 | A | G | 7.3%
| 140.2
/ 4.2
| 55 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (27/24); new base G (2/2); total (29/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 |
CGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAG > minE/2531546‑2531678
|
cGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGAt > 1:610997/1‑71 (MQ=255)
gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1157188/1‑71 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACAATTGGTGATGGGATCGa < 1:205095/71‑1 (MQ=255)
gTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg > 1:603254/1‑71 (MQ=255)
gAACATCTGGGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1742911/56‑1 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:198460/56‑1 (MQ=255)
aaCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATa < 1:1178738/68‑1 (MQ=255)
cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaaa > 1:1978680/1‑68 (MQ=255)
tCTGGGTGGCGCGCGTCACGATATCGATATGACCATTGGTaa < 1:252961/42‑1 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1666656/51‑1 (MQ=255)
tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:160429/1‑71 (MQ=255)
cGTGTCGCGAGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg < 1:2011464/69‑1 (MQ=255)
gTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttt < 1:1036816/71‑1 (MQ=255)
tGGCGCGCGTCACGATATCGATATGACCATTGGTa < 1:1527863/35‑1 (MQ=255)
tGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:137778/45‑1 (MQ=255)
tGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:1456016/71‑1 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg > 1:2073313/1‑71 (MQ=255)
gcgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:2107791/1‑57 (MQ=255)
cgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt < 1:1365663/71‑1 (MQ=255)
gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc > 1:524569/1‑46 (MQ=255)
gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc > 1:994376/1‑46 (MQ=255)
cgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa < 1:1844277/70‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg < 1:397179/45‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca < 1:1823148/71‑1 (MQ=255)
cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttttt > 1:1091958/1‑62 (MQ=255)
cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:572944/1‑66 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:1920187/1‑71 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac < 1:926144/71‑1 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac < 1:871002/71‑1 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:521012/1‑71 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaa > 1:1011184/1‑67 (MQ=255)
gatatcgatatGACCATTGGTAATGGGATCGAAAg > 1:1659635/1‑35 (MQ=255)
atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGAt > 1:624736/1‑43 (MQ=255)
tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:5634/64‑1 (MQ=255)
tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc > 1:768265/1‑70 (MQ=255)
tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa < 1:2126304/71‑1 (MQ=255)
tatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa < 1:1213639/71‑1 (MQ=255)
tatagatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt > 1:1756603/1‑69 (MQ=255)
atcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:2096652/1‑71 (MQ=255)
gatatGAGCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1261719/50‑1 (MQ=255)
gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCa > 1:975820/1‑67 (MQ=255)
gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc < 1:954322/71‑1 (MQ=255)
atGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGtt > 1:839521/1‑71 (MQ=255)
tGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:62130/36‑1 (MQ=255)
aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca > 1:475426/1‑55 (MQ=255)
aTTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa < 1:903687/49‑1 (MQ=255)
ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:435397/67‑1 (MQ=255)
tGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:666936/1‑51 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:465735/1‑50 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:268945/1‑50 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:1244307/1‑50 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaa > 1:2184536/1‑50 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAAt > 1:356305/1‑57 (MQ=255)
ggTAATGGGATCGAAAGTACCCCGATAAATCGCCCGTTTTTGCATAACAACCTCAAt > 1:2062609/1‑57 (MQ=255)
tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGttt < 1:1488684/61‑1 (MQ=255)
tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1285584/63‑1 (MQ=255)
tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAg < 1:157506/71‑1 (MQ=255)
aaTGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:694589/1‑53 (MQ=255)
|
CGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAG > minE/2531546‑2531678
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
