Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,531,609 |
A→G |
64.2% |
I14I (ATT→ATC) |
coaD ← |
pantetheine‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,531,609 | 0 | A | G | 64.2%
| 70.1
/ 68.0
| 67 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (13/11); new base G (18/25); total (31/36) |
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01 |
TCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAGG > minE/2531545‑2531679
|
tCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGa > 1:205679/1‑71 (MQ=255)
cGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGAt > 1:3831027/1‑71 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:751677/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3062264/71‑1 (MQ=255)
aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAcc < 1:501099/71‑1 (MQ=255)
aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTAc < 1:3327675/70‑1 (MQ=255)
gTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCg > 1:2033381/1‑71 (MQ=255)
gATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCgg < 1:2571571/70‑1 (MQ=255)
aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:1565558/59‑1 (MQ=255)
aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:532214/59‑1 (MQ=255)
aTCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2231608/59‑1 (MQ=255)
tCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa < 1:1768260/71‑1 (MQ=255)
tCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa < 1:1773287/71‑1 (MQ=255)
cGAAGATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATc > 1:1087639/1‑55 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:2202748/1‑70 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:3176528/1‑70 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:1865324/1‑70 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:1234865/1‑70 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATa > 1:3866122/1‑70 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:2514677/70‑1 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:1179245/70‑1 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:1740743/70‑1 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATggg < 1:2236288/51‑1 (MQ=255)
aaCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa < 1:450704/69‑1 (MQ=255)
aCATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAAt > 1:3062011/1‑70 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATGTGACCATTGGTGATGGGATCGAAAGTa < 1:2188996/56‑1 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:682700/1‑71 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:3840741/1‑71 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:3005436/1‑71 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:2939177/1‑71 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGcc > 1:371847/1‑71 (MQ=255)
cTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGccc > 1:1396730/1‑71 (MQ=255)
tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:619109/1‑71 (MQ=255)
atcGTGACGCGCGCCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:3579149/3‑71 (MQ=255)
atcGTGACGCGCGCCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:1712729/3‑71 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttttt > 1:2219614/1‑70 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg > 1:1896799/1‑71 (MQ=255)
cgcgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt < 1:2091960/71‑1 (MQ=255)
gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:3744273/63‑1 (MQ=255)
gcgTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1694755/63‑1 (MQ=255)
gcgACACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:2004298/63‑1 (MQ=255)
tCACGATATCGATATGACCATTGTTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:3917272/62‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaaca < 1:1560531/71‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:375803/70‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaac < 1:2569731/70‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:938122/50‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:2196123/62‑1 (MQ=255)
aCGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:387747/1‑71 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATaacaac > 1:3585132/1‑71 (MQ=255)
aCGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGGTTTTGCATaacaac > 1:2078606/1‑71 (MQ=255)
atatcgatatGACCATTGGTAATTGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:962699/71‑1 (MQ=255)
atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:3380911/71‑1 (MQ=255)
atatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTc < 1:1883986/71‑1 (MQ=255)
tcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:1392505/52‑1 (MQ=255)
cgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa > 1:4044222/1‑57 (MQ=255)
gatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:1194817/1‑68 (MQ=255)
gatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCaa > 1:1602960/1‑68 (MQ=255)
gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc < 1:1666520/71‑1 (MQ=255)
gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc > 1:490565/1‑71 (MQ=255)
gatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGc > 1:1885637/1‑71 (MQ=255)
tGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:663493/1‑53 (MQ=255)
tGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCAt > 1:2593069/1‑53 (MQ=255)
aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1721973/71‑1 (MQ=255)
aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:594714/71‑1 (MQ=255)
aCCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:788481/71‑1 (MQ=255)
aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTc < 1:1818370/71‑1 (MQ=255)
gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTTCATAACAACCTCAATGCGTTTTCGGTGGc < 1:2386384/70‑1 (MQ=255)
aaTGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAgg > 1:2855933/1‑71 (MQ=255)
|
TCGCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCAGG > minE/2531545‑2531679
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
