Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,531,609 |
A→G |
24.0% |
I14I (ATT→ATC) |
coaD ← |
pantetheine‑phosphate adenylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,531,609 | 0 | A | G | 24.0%
| 138.3
/ 47.3
| 75 | I14I (ATT→ATC) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (24/33); new base G (2/16); total (26/49) |
| Fisher's exact test for biased strand distribution p-value = 2.19e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 |
GCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCA > minE/2531547‑2531677
|
gacAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1902855/3‑71 (MQ=255)
gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGTGATc > 1:1556255/1‑71 (MQ=255)
gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGAt > 1:1672744/1‑70 (MQ=255)
gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:3415800/1‑71 (MQ=255)
gCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATc > 1:1561243/1‑71 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2411482/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:2371389/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3392291/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3809034/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3423360/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3438025/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:3757157/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:278756/71‑1 (MQ=255)
cAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:3121255/71‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:3705385/70‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:2254049/70‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:3078958/70‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGaa < 1:536904/70‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1640663/69‑1 (MQ=255)
gAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:3196299/69‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAg < 1:951877/71‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:3808997/71‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:383703/71‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:2138914/71‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:332216/71‑1 (MQ=255)
aaTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAg < 1:448639/71‑1 (MQ=255)
aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:3798511/1‑69 (MQ=255)
aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:3106751/1‑69 (MQ=255)
aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:1122306/1‑69 (MQ=255)
aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGaaa > 1:1941679/1‑69 (MQ=255)
aTAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGt < 1:550391/71‑1 (MQ=255)
tAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGa < 1:1593202/66‑1 (MQ=255)
aCGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:4005919/64‑1 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTaa > 1:503985/1‑48 (MQ=255)
cGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGAATCGAAAGTACCCGGATa > 1:4053039/1‑70 (MQ=255)
gAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTGATGGGATCGAAAGTACCCGGATaa > 1:439961/1‑70 (MQ=255)
cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:253011/1‑69 (MQ=255)
cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:1403806/1‑69 (MQ=255)
cATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:3151919/1‑69 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:1516068/51‑1 (MQ=255)
tCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGa < 1:7840/51‑1 (MQ=255)
tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:2258684/1‑71 (MQ=255)
tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:1002033/1‑71 (MQ=255)
tGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCg > 1:3993322/1‑71 (MQ=255)
cGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtt < 1:3448548/71‑1 (MQ=255)
tGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGtttt < 1:3427920/71‑1 (MQ=255)
ggCGCGCGTCACGATATCGATATGACCATTGGTaa > 1:3701942/1‑35 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:2100634/71‑1 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:874319/71‑1 (MQ=255)
gcgcgcGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTg < 1:3537027/71‑1 (MQ=255)
cgTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:236997/1‑70 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2729264/50‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2189321/50‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:264368/50‑1 (MQ=255)
tCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:3998121/50‑1 (MQ=255)
cACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATaa < 1:2349042/49‑1 (MQ=255)
cgatatcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAAcc < 1:1432741/71‑1 (MQ=255)
cgatatcgatatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAAcc < 1:2218775/71‑1 (MQ=255)
gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:2546353/71‑1 (MQ=255)
gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:2119231/71‑1 (MQ=255)
gatatcgatatGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCt < 1:3144935/71‑1 (MQ=255)
tatGACCATTGGTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGt < 1:2734720/71‑1 (MQ=255)
aCCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:1286116/1‑36 (MQ=255)
aCCATTGGTAATGGGATCGAAAGTACCCGGATAAAt > 1:2555041/1‑36 (MQ=255)
aCCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGttt > 1:1652642/1‑45 (MQ=255)
aCCATTGGTAATGGGATCGAAAATACCCGGATAAAt > 1:3289777/1‑36 (MQ=255)
cATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCgg < 1:3842631/71‑1 (MQ=255)
ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTg < 1:2802686/71‑1 (MQ=255)
ttGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTg < 1:2468972/71‑1 (MQ=255)
ggTGATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTgg > 1:2020792/1‑70 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:2979884/1‑46 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATa > 1:3554480/1‑46 (MQ=255)
ggTAATGGGATCGAAAGTACCCGGATAAATCGCCAGTTTTTGCATa > 1:984185/1‑46 (MQ=255)
gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCa < 1:2721507/71‑1 (MQ=255)
gTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCa < 1:1204361/71‑1 (MQ=255)
tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa < 1:555529/42‑1 (MQ=255)
tAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCa < 1:1776618/42‑1 (MQ=255)
|
GCCAGAATAACGTGATCGAACATCTGCGTGGCGCGCGTCACGATATCGATATGACCATTGGTAATGGGATCGAAAGTACCCGGATAAATCGCCCGTTTTTGCATAACAACCTCAATGCGTTTTCGGTGGCA > minE/2531547‑2531677
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
