Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
546,484 |
G→T |
27.5% |
G194V (GGC→GTC) |
fdrA → |
predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 546,484 | 0 | G | T | 27.5%
| 123.0
/ 29.1
| 58 | G194V (GGC→GTC) | fdrA | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base T (9/7); total (31/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
GGGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCA > W3110S.gb/546416‑546551
|
ggggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGc > 1:3385653/1‑70 (MQ=255)
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa > 1:110816/1‑71 (MQ=255)
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa > 1:2697521/1‑71 (MQ=255)
ccGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:4320698/1‑71 (MQ=255)
ggACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATAtt < 1:1281850/70‑1 (MQ=255)
ggACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATAtt < 1:3177212/70‑1 (MQ=255)
aCTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGc < 1:3107056/71‑1 (MQ=255)
aCTGAGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGc > 1:3453102/1‑71 (MQ=255)
ggTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt < 1:1068181/71‑1 (MQ=255)
gTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAtt < 1:1355502/71‑1 (MQ=255)
tACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTg < 1:2164361/71‑1 (MQ=255)
aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTgg > 1:2547745/1‑71 (MQ=255)
cGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgc > 1:2335188/1‑71 (MQ=255)
tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCAt > 1:1002127/1‑65 (MQ=255)
cGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt > 1:900321/1‑71 (MQ=255)
cGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCt > 1:52667/1‑71 (MQ=255)
gatgatTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:1054116/1‑71 (MQ=255)
gatgatTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:2056196/1‑71 (MQ=255)
atTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCgg < 1:2174842/71‑1 (MQ=255)
ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGt < 1:1424265/71‑1 (MQ=255)
ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTg > 1:3869234/1‑60 (MQ=255)
ttGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTg > 1:1156363/1‑60 (MQ=255)
gCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTAc > 1:1116485/1‑71 (MQ=255)
ggCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCgg < 1:2475191/71‑1 (MQ=255)
ggCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCgg > 1:4093729/1‑71 (MQ=255)
cacaCCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATT‑GCGCTTcc < 1:501204/60‑1 (MQ=255)
acacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGc > 1:2512085/1‑36 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAat < 1:3573174/38‑1 (MQ=255)
acCGCTGGCTTTTGCTAACGTGATGCCTGAAGGCAATATTGGCGTCATTGGCGCTTCCGGt < 1:1134241/61‑1 (MQ=255)
acCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAata < 1:2543556/38‑1 (MQ=255)
ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:2153387/1‑38 (MQ=255)
ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGAtt > 1:814236/1‑69 (MQ=255)
cGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:1994098/1‑71 (MQ=255)
cGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:3820519/1‑71 (MQ=255)
gCTGGCTTTTGCTAATGTGATGCCGGAAGGCAata > 1:2553682/1‑35 (MQ=255)
tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGt < 1:2747223/56‑1 (MQ=255)
tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg > 1:3669981/1‑71 (MQ=255)
tGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGGCATTGGCGCTTCCGGt < 1:2752392/56‑1 (MQ=255)
gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTAc > 1:1637088/1‑56 (MQ=255)
gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg < 1:1462263/69‑1 (MQ=255)
gCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAg < 1:1707451/69‑1 (MQ=255)
cTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCtg < 1:2608597/71‑1 (MQ=255)
ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCtg < 1:3672948/68‑1 (MQ=255)
gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCtt > 1:2777879/1‑43 (MQ=255)
gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTcc < 1:3493661/45‑1 (MQ=255)
gCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc > 1:764853/1‑71 (MQ=255)
aCGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg > 1:4264197/1‑68 (MQ=255)
cGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGa < 1:494389/71‑1 (MQ=255)
gTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc > 1:2695782/1‑65 (MQ=255)
tGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTc < 1:310171/64‑1 (MQ=255)
aTGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCTGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgc > 1:1138828/1‑71 (MQ=255)
tGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgcg < 1:2431813/71‑1 (MQ=255)
gCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTgcg > 1:1479117/1‑70 (MQ=255)
ggAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg < 1:3356516/71‑1 (MQ=255)
ggAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg < 1:2984389/71‑1 (MQ=255)
ggAAGGCAATATTGGAGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTgg > 1:1306368/1‑71 (MQ=255)
gAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGc < 1:3449045/71‑1 (MQ=255)
aaGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCa < 1:1725572/71‑1 (MQ=255)
|
GGGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGCA > W3110S.gb/546416‑546551
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
