Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
546,484 |
G→T |
27.4% |
G194V (GGC→GTC) |
fdrA → |
predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 546,484 | 0 | G | T | 27.4%
| 92.4
/ 17.5
| 40 | G194V (GGC→GTC) | fdrA | predicted acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base T (5/6); total (19/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGC > W3110S.gb/546417‑546550
|
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCaa < 1:222060/71‑1 (MQ=255)
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3276909/71‑1 (MQ=255)
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:254273/71‑1 (MQ=255)
gggCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3431685/71‑1 (MQ=255)
gCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt > 1:159013/1‑70 (MQ=255)
cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:15210/69‑1 (MQ=255)
cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:1906938/69‑1 (MQ=255)
cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGt < 1:2149163/69‑1 (MQ=255)
cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCa > 1:2020928/1‑71 (MQ=255)
cGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCa > 1:2147017/1‑71 (MQ=255)
aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAatat > 1:3385644/1‑59 (MQ=255)
aCGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTgg > 1:173310/1‑71 (MQ=255)
cGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTgg > 1:3457028/1‑70 (MQ=255)
tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:875387/1‑71 (MQ=255)
tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:4047683/1‑71 (MQ=255)
tCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGgcgc > 1:3190309/1‑71 (MQ=255)
tGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTa > 1:725687/1‑71 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2227707/56‑1 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2173432/56‑1 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:553698/56‑1 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCt < 1:2792525/56‑1 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3320809/37‑1 (MQ=255)
cacCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCaa < 1:3746026/37‑1 (MQ=255)
acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt > 1:1071904/1‑48 (MQ=255)
acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAt > 1:1751721/1‑48 (MQ=255)
acCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCAATGGCGCTTCCGGTACCGGGATTc < 1:3047062/71‑1 (MQ=255)
ccGCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCtt > 1:2699728/1‑55 (MQ=255)
ccGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTc < 1:3415748/45‑1 (MQ=255)
gCTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAgg < 1:655255/71‑1 (MQ=255)
cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:3785740/1‑69 (MQ=255)
cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAg > 1:124941/1‑69 (MQ=255)
cTGGCTTTTGCTAACGTGATGCCGGAAGTCAATATTGGCGTCATTGGCGCTTCAGGTACCGGGATTCAg > 1:2756947/1‑69 (MQ=255)
ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTgtgt > 1:4719/1‑71 (MQ=255)
ttGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTgtgt > 1:2459002/1‑71 (MQ=255)
cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:2864417/71‑1 (MQ=255)
cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:3801206/71‑1 (MQ=255)
cTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCg < 1:2345192/71‑1 (MQ=255)
aCGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCa < 1:2723603/70‑1 (MQ=255)
aCGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCa < 1:2268944/70‑1 (MQ=255)
ccGGAAGTCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGa > 1:3710716/1‑64 (MQ=255)
ggCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGc < 1:1469151/68‑1 (MQ=255)
|
GGGCCGGACTGCGGTACGTCGATGATTGCCGGCACACCGCTGGCTTTTGCTAACGTGATGCCGGAAGGCAATATTGGCGTCATTGGCGCTTCCGGTACCGGGATTCAGGAGCTGTGTTCGCAGATTGCGCTGGC > W3110S.gb/546417‑546550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
