Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,381,932 |
Δ1 bp |
coding (331/759 nt) |
yfbB ← |
predicted peptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,381,930 | 0 | C | . | 100.0%
| 93.6
/ NA
| 24 | coding (333/759 nt) | yfbB | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (4/20); total (4/20) |
GCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCA > W3110S.gb/2381863‑2381974
|
gCTGCACCCTTTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:825019/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:1010640/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:765180/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:1766680/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:2569549/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:1804612/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:1889765/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:2172563/71‑3 (MQ=255)
gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:254251/71‑3 (MQ=255)
cATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccg < 1:2993850/62‑2 (MQ=255)
ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATa < 1:417034/70‑1 (MQ=255)
ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATa < 1:4045767/70‑1 (MQ=255)
aTCGGAACGCTGACGTTCCGCACGTTGTTCATCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:304787/59‑1 (MQ=255)
aTCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:520563/59‑1 (MQ=255)
cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCca > 1:2726218/1‑70 (MQ=255)
ttCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGccgc < 1:3368234/40‑3 (MQ=255)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:943913/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:3705588/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:78697/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:3248031/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:310151/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:2608130/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:2400004/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTc < 1:2391169/41‑1 (MQ=39)
cGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGcc < 1:1749432/70‑1 (MQ=255)
cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa > 1:1551465/1‑41 (MQ=39)
cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa > 1:1484163/1‑41 (MQ=39)
cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGa > 1:844467/1‑41 (MQ=39)
cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCt < 1:2627550/71‑1 (MQ=255)
cACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCt < 1:1778831/71‑1 (MQ=255)
ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa < 1:3175350/71‑1 (MQ=255)
ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa < 1:3908481/71‑1 (MQ=255)
ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa < 1:462441/71‑1 (MQ=255)
ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa < 1:942433/71‑1 (MQ=255)
ttgttCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCa < 1:1042240/71‑1 (MQ=255)
|
GCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCA > W3110S.gb/2381863‑2381974
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
