Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F8 I0 R2	296	77.6	4260404	88.1%	3753415	61.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,971,396	T→C	28.4%	V71A (GTG→GCG)	serB →	3‑phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,971,396	0	T	C	28.4%	107.9 / 58.7	74	V71A (GTG→GCG)	serB	3‑phosphoserine phosphatase
Reads supporting (aligned to +/- strand): ref base T (14/39); new base C (10/11); total (24/50)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

ATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGA  >  minE/2971327‑2971462

aTAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTg                                                                   >  1:734801/1‑71 (MQ=255)

aTAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTg                                                                   >  1:283161/1‑71 (MQ=255)

aTAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTg                                                                   >  1:2485468/1‑71 (MQ=255)

  aaaCAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCgg                                                                  >  1:789965/1‑70 (MQ=255)

  aaaCAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCgg                                                                  >  1:3501026/1‑70 (MQ=255)

    acaacGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTgg                                                                  >  1:2287300/1‑68 (MQ=255)

     caacGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTgg                                                                  >  1:151044/1‑67 (MQ=255)

     caacGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGa                                                              <  1:3046416/71‑1 (MQ=255)

     caacGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGACTGGTGCGCGGAAGa                                                              <  1:2222921/71‑1 (MQ=255)

      aacGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAt                                                             >  1:302939/1‑71 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCTTGGAAGAtt                                                            <  1:2716858/71‑1 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAtt                                                            <  1:1098208/71‑1 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAtt                                                            <  1:3565546/71‑1 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAtt                                                            <  1:3498256/71‑1 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAtt                                                            <  1:2289628/71‑1 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAt                                                             >  1:3388446/1‑70 (MQ=255)

       acGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAt                                                             >  1:3082391/1‑70 (MQ=255)

        cGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTa                                                           <  1:3942415/71‑1 (MQ=255)

               cccAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATc                                                         <  1:1286979/66‑1 (MQ=255)

                     aCCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCg                                               <  1:30714/70‑1 (MQ=255)

                       cAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAg                                                       >  1:728032/1‑60 (MQ=255)

                       cAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAg                                                       >  1:2494904/1‑60 (MQ=255)

                        agagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTg                                           >  1:171226/1‑71 (MQ=255)

                        agagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTg                                           >  1:1182101/1‑71 (MQ=255)

                         gagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAt                                                             <  1:1188015/52‑1 (MQ=255)

                         gagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTgg                                          <  1:2938993/71‑1 (MQ=255)

                         gagCAAACTGGGTGCGGCGATGGTGATTGTT‑‑‑GCCTGGTGCGTGGAAGAt                                                             <  1:4213070/49‑1 (MQ=37)

                           gCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTgg                                          >  1:329391/1‑69 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAg                                       >  1:2173827/1‑71 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAg                                       >  1:3295890/1‑71 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAg                                       >  1:3244136/1‑71 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAg                                       >  1:1708390/1‑71 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGAAg                                       >  1:2281378/1‑71 (MQ=255)

                            cAAACTGGGTGCGGCGATGGTGATTGTCGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAg                                       <  1:3852059/71‑1 (MQ=255)

                             aaaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAg                                       <  1:4060319/70‑1 (MQ=255)

                              aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGt                                     <  1:1425864/71‑1 (MQ=255)

                              aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGt                                     <  1:2884615/71‑1 (MQ=255)

                                 tGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTtca                                  >  1:4047726/1‑71 (MQ=255)

                                   ggTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcac                                 <  1:1946385/70‑1 (MQ=255)

                                   ggTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcac                                 <  1:2220360/70‑1 (MQ=255)

                                   ggTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcac                                 <  1:2280042/70‑1 (MQ=255)

                                   ggTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGAATATCAGGTGATTCGTCTGGCAGGTtcac                                 <  1:1773869/70‑1 (MQ=255)

                                        ggcgATGGTGATTGTTGCCGCCTGGTGCGTGgatgat                                                             <  1:4149118/37‑1 (MQ=255)

                                        ggcgATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCg                           <  1:491894/71‑1 (MQ=255)

                                          cgATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCa                         <  1:2065847/71‑1 (MQ=255)

                                          cgATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCa                         >  1:1156943/1‑71 (MQ=255)

                                          cgATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCa                         <  1:1676231/71‑1 (MQ=255)

                                             tGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAg                                       <  1:2599481/54‑1 (MQ=255)

                                             tGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGc                          <  1:2078224/67‑1 (MQ=255)

                                               gTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTATGGCAGGTTCACTCACCGCACGGGc                    <  1:4162751/71‑1 (MQ=255)

                                                  aTTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGc                                         <  1:3194760/47‑1 (MQ=255)

                                                  aTTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGc                                         <  1:267498/47‑1 (MQ=255)

                                                  aTTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTa                  <  1:53667/70‑1 (MQ=255)

                                                  aTTGTTGCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTa                  <  1:1096078/70‑1 (MQ=255)

                                                   ttgttgCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACggg                     <  1:1725065/66‑1 (MQ=255)

                                                   ttgttgCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACggg                     <  1:1398088/66‑1 (MQ=255)

                                                   ttgttgCCGCCTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTa                  <  1:1996039/69‑1 (MQ=255)

                                                         ccgcgTGGTGGGTGGAAGATTATCAGGTGATTCGTCt                                            <  1:4259806/37‑1 (MQ=38)

                                                         ccgccTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCt           <  1:1239823/70‑1 (MQ=255)

                                                            ccTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCAc                        >  1:3567752/1‑54 (MQ=255)

                                                            ccTGGTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCAc                        >  1:2931361/1‑54 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:816385/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:1116524/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:3185869/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:135380/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:2588319/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTac                 <  1:2214399/60‑1 (MQ=255)

                                                             cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGGTCACTCACCGCACGGGCTac                 <  1:1548799/60‑1 (MQ=255)

                                                                gTGCGCGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCa     <  1:3608997/69‑1 (MQ=255)

                                                                 tGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGa  <  1:4053484/71‑1 (MQ=255)

                                                                 tGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGa  <  1:1153265/71‑1 (MQ=255)

                                                                 tGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGa  <  1:3524021/71‑1 (MQ=255)

                                                                 tGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGa  <  1:1866788/71‑1 (MQ=255)

                                                                 tGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGa  <  1:762311/71‑1 (MQ=255)

ATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGA  >  minE/2971327‑2971462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A