Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,000,186 |
G→A |
G179D (GGT→GAT) |
ydgD → |
predicted peptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,000,186 | 0 | G | A | 100.0%
| 155.8
/ NA
| 53 | G179D (GGT→GAT) | ydgD | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/30); total (23/30) |
CATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTG > minE/1000121‑1000252
|
cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGt > 1:929518/1‑70 (MQ=255)
cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa < 1:2295206/71‑1 (MQ=255)
cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa < 1:2291653/71‑1 (MQ=255)
ttACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTaaa < 1:987012/71‑1 (MQ=255)
aCGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAg > 1:2556745/1‑70 (MQ=255)
cgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACt < 1:661553/71‑1 (MQ=255)
ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa < 1:834231/61‑1 (MQ=255)
ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACt > 1:1678831/1‑69 (MQ=255)
tgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcag > 1:1088118/1‑71 (MQ=255)
ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggc < 1:737512/71‑1 (MQ=255)
cgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggca < 1:410305/71‑1 (MQ=255)
cgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggca < 1:1734593/71‑1 (MQ=255)
ttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTaaa > 1:2916831/1‑57 (MQ=255)
aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCaggcaggc > 1:1038845/1‑70 (MQ=255)
aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCaggcaggc < 1:1394531/70‑1 (MQ=255)
tttGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTa < 1:2120594/71‑1 (MQ=255)
gggAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg < 1:2773570/71‑1 (MQ=255)
agaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTca < 1:2641702/54‑1 (MQ=255)
agaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTca < 1:855649/54‑1 (MQ=255)
aTAAAGCCGCGCTTACCGCCTCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg > 1:886121/1‑66 (MQ=255)
aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg > 1:2797670/1‑66 (MQ=255)
aGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcatc < 1:159264/71‑1 (MQ=255)
gcTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa > 1:2353750/1‑71 (MQ=255)
gcTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa > 1:1231365/1‑71 (MQ=255)
cTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAg < 1:2281060/58‑1 (MQ=255)
cTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATAc > 1:1446157/1‑71 (MQ=255)
cTTACCGCCGCATTAAAAGCGGAAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAg < 1:2178122/58‑1 (MQ=255)
aCCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa > 1:142093/1‑67 (MQ=255)
ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGaa > 1:1425197/1‑53 (MQ=255)
ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGtt < 1:1442586/70‑1 (MQ=255)
ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGtt < 1:1056294/70‑1 (MQ=255)
ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTg < 1:967796/71‑1 (MQ=255)
cgccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTg > 1:92718/1‑70 (MQ=255)
ccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTa > 1:2338753/1‑70 (MQ=255)
ccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTAc < 1:1088861/71‑1 (MQ=255)
aTTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGAt > 1:2960120/1‑58 (MQ=255)
aTTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGt > 1:2625384/1‑70 (MQ=255)
aaaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcat > 1:2378516/1‑49 (MQ=255)
aaaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat > 1:1895677/1‑70 (MQ=255)
aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTGGATACGTTg < 1:2083442/60‑1 (MQ=255)
aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGAt < 1:2502114/54‑1 (MQ=255)
aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcatc < 1:59619/70‑1 (MQ=255)
aaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaa < 1:1362202/71‑1 (MQ=255)
aGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt > 1:2750045/1‑56 (MQ=255)
aGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaaa < 1:2939294/71‑1 (MQ=255)
gCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaaaa > 1:1078946/1‑71 (MQ=255)
cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcatat < 1:2088803/46‑3 (MQ=255)
cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtct < 1:507079/46‑1 (MQ=255)
cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtct < 1:2970965/46‑1 (MQ=255)
cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCg < 1:1799653/48‑1 (MQ=255)
gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc < 1:1136121/45‑1 (MQ=255)
gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtg > 1:1732842/1‑71 (MQ=255)
gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtg > 1:1736466/1‑71 (MQ=255)
|
CATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTG > minE/1000121‑1000252
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
