Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,695,295 |
G→A |
Q239* (CAG→TAG) |
yfhQ ← |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,695,295 | 0 | G | A | 100.0%
| 106.3
/ NA
| 36 | Q239* (CAG→TAG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/21); total (15/21) |
GGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGT > minE/1695227‑1695361
|
ggCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctact > 1:2392975/1‑71 (MQ=255)
aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCaa > 1:1490966/1‑71 (MQ=255)
aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCa < 1:2005172/70‑1 (MQ=255)
gACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAg > 1:991446/1‑70 (MQ=255)
gACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAg > 1:2027488/1‑70 (MQ=255)
gACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGa < 1:78373/71‑1 (MQ=255)
gCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGc < 1:1763265/71‑1 (MQ=255)
gCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGc < 1:330064/71‑1 (MQ=255)
ttACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCa < 1:284234/70‑1 (MQ=255)
ttACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAg < 1:3020052/71‑1 (MQ=255)
tACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAg > 1:2925050/1‑70 (MQ=255)
tACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAg > 1:2888929/1‑70 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATa > 1:1483112/1‑61 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGaa > 1:2167469/1‑71 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGa < 1:2372249/70‑1 (MQ=255)
gTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGcc > 1:2252470/1‑63 (MQ=255)
gTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGcc > 1:2386551/1‑63 (MQ=255)
gCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAg < 1:2484373/48‑1 (MQ=255)
aaCGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAgg < 1:1008848/66‑1 (MQ=255)
aCGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCa > 1:77124/1‑51 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt < 1:168646/71‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGAATATTCAACTCCt > 1:2121485/1‑71 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATTTTCAACTCCTgg > 1:2223534/1‑71 (MQ=255)
cGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCt < 1:1393582/70‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:1807110/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:429389/71‑1 (MQ=255)
gCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTc < 1:2849424/71‑1 (MQ=255)
ccTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc < 1:2971369/71‑1 (MQ=255)
ccTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc < 1:2999542/71‑1 (MQ=255)
ccTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc > 1:387119/1‑71 (MQ=255)
tGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCtt < 1:1782123/65‑1 (MQ=255)
gTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCgcgc > 1:1923412/1‑43 (MQ=255)
gTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCgg < 1:140912/69‑1 (MQ=255)
tttATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgc < 1:2312185/67‑1 (MQ=255)
tATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCAc < 1:2316169/69‑1 (MQ=255)
tATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCAc < 1:1674644/69‑1 (MQ=255)
tCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGt > 1:438888/1‑70 (MQ=255)
|
GGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGGGT > minE/1695227‑1695361
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
