Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R1
|
122 |
92.7 |
4382561 |
94.3% |
4132755 |
66.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,695,295 |
G→A |
Q239* (CAG→TAG) |
yfhQ ← |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,695,295 | 0 | G | A | 100.0%
| 145.8
/ NA
| 44 | Q239* (CAG→TAG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/24); total (20/24) |
CCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGC > minE/1695229‑1695355
|
ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:1167511/71‑1 (MQ=255)
ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2934092/71‑1 (MQ=255)
ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2864032/71‑1 (MQ=255)
ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2355038/71‑1 (MQ=255)
ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:1553074/71‑1 (MQ=255)
cTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:1237486/70‑1 (MQ=255)
cTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:524796/70‑1 (MQ=255)
cTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2076397/70‑1 (MQ=255)
aCGCGTTGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGaa > 1:179802/1‑71 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGaa > 1:179323/1‑71 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGaa > 1:2094377/1‑71 (MQ=255)
aCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGaa > 1:3338682/1‑71 (MQ=255)
gcgTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:3320862/53‑1 (MQ=255)
cgTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:1415911/52‑1 (MQ=255)
tCAGGCAACGGCTGTTATTCAGCCTTGTTACCTTTATTCtactgc < 1:162582/45‑1 (MQ=38)
aGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:4048747/43‑1 (MQ=255)
aGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2219561/43‑1 (MQ=255)
gCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:3494190/41‑1 (MQ=255)
gCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:894494/41‑1 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:4037748/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:972673/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:3890131/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:3562439/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:938262/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:3309348/1‑71 (MQ=255)
cAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat > 1:1211951/1‑71 (MQ=255)
aCGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:1645802/38‑1 (MQ=255)
cGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:3504872/37‑1 (MQ=255)
cGGCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:2976491/37‑1 (MQ=255)
gCTGTTATTCGGCCTTGTTACCTTTATTCtactgc < 1:587898/35‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:851354/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:3476080/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:2765277/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:3533227/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:4225419/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:418376/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:4140065/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:3659062/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:917084/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:3897605/59‑1 (MQ=255)
ttATTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGa < 1:4024780/59‑1 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCttg > 1:1723566/1‑69 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg > 1:804830/1‑71 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg > 1:3253810/1‑71 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg > 1:3106268/1‑71 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg > 1:3058672/1‑71 (MQ=255)
aTTCGGCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTg > 1:3648919/1‑70 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:3140452/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:1248034/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:660218/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:1388704/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:4329054/71‑1 (MQ=255)
ggCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt < 1:517762/71‑1 (MQ=255)
gCCTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCTCAGGATATTCAACTCCTGGCttt < 1:1174700/70‑1 (MQ=255)
cTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCg > 1:3335520/1‑71 (MQ=255)
cTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCg > 1:3318384/1‑71 (MQ=255)
cTTGTTACCTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCg > 1:3897726/1‑71 (MQ=255)
aCCTTTATTCTACTGCTAAATAGAAGCCAGAATCCCGCTCATGa < 1:1880650/44‑1 (MQ=25)
ccTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcgc < 1:3982546/71‑1 (MQ=255)
ccTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcg < 1:4297715/70‑1 (MQ=255)
ccTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcg < 1:4194741/70‑1 (MQ=255)
ccTTTATTCTACTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcg < 1:2537571/70‑1 (MQ=255)
ccTTTATTCTACTGCTCAATAGAAGACAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgcgcgc < 1:3561557/71‑1 (MQ=255)
|
CCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGC > minE/1695229‑1695355
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
